BRENDA - Enzyme Database

Function and structure of rat hepatic coproporphyrinogen oxidase

Sorianello, E.M.; Mazzetti, M.B.; Comp. Biochem. Physiol. B 127, 155-164 (2000)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.3.3.3
L-alpha-phosphatidylcholine
activation in sonicated mitochondria up to 150%
Rattus sp.
1.3.3.3
phosphatidate
activation in sonicated mitochondria up to 150%
Rattus sp.
1.3.3.3
phosphatidyl glycerol
activation in intact and sonicated mitochondria up to 135%
Rattus sp.
1.3.3.3
Triton X-100
concentration-dependent activation up to 2.8fold
Rattus sp.
1.3.3.3
Tween 20
concentration-dependent activation up to 152%
Rattus sp.
General Stability
EC Number
General Stability
Organism
1.3.3.3
purified enzyme rather unstable, 2 days
Rattus sp.
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.3.3.3
diethyl dicarbonate
concentration-dependent inhibition, complete inactivation at 50 mM
Rattus sp.
1.3.3.3
KCl
70% inhibition at 1 M
Rattus sp.
1.3.3.3
N-bromosuccinimide
concentration-dependent inhibition, complete inactivation at 50 mM
Rattus sp.
1.3.3.3
NaCl
60% inhibition at 1 M
Rattus sp.
1.3.3.3
Phenylglyoxal
concentration-dependent inhibition, complete inactivation at 50 mM
Rattus sp.
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.3.3.3
mitochondrion
located within intermembrane space
Rattus sp.
5739
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.3.3.3
38000
-
2 * 38000, SDS-PAGE
Rattus sp.
1.3.3.3
77000
-
minimum catalytic activity, also aggregation to 154000 kDa catalytic activity, gel filtration
Rattus sp.
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.3.3.3
coproporphyrinogen-III + O2
Rattus sp.
6th enzyme in heme biosynthetic pathway
protoporphyrinogen-IX + CO2
-
Rattus sp.
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.3.3.3
Rattus sp.
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.3.3.3
to homogeneity, chromatography techniques
Rattus sp.
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.3.3.3
liver
-
Rattus sp.
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.3.3.3
0.009
-
-
Rattus sp.
Storage Stability
EC Number
Storage Stability
Organism
1.3.3.3
-20°C, 30-65% ammonium sulfate precipitate, 2 months
Rattus sp.
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.3.3.3
coproporphyrinogen-III + O2
sixths enzyme in heme biosynthetic pathway
390943
Rattus sp.
protoporphyrinogen-IX + CO2
-
390943
Rattus sp.
?
1.3.3.3
coproporphyrinogen-III + O2
6th enzyme in heme biosynthetic pathway
390943
Rattus sp.
protoporphyrinogen-IX + CO2
-
390943
Rattus sp.
?
Subunits
EC Number
Subunits
Commentary
Organism
1.3.3.3
dimer
2 * 38000, SDS-PAGE
Rattus sp.
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.3.3.3
L-alpha-phosphatidylcholine
activation in sonicated mitochondria up to 150%
Rattus sp.
1.3.3.3
phosphatidate
activation in sonicated mitochondria up to 150%
Rattus sp.
1.3.3.3
phosphatidyl glycerol
activation in intact and sonicated mitochondria up to 135%
Rattus sp.
1.3.3.3
Triton X-100
concentration-dependent activation up to 2.8fold
Rattus sp.
1.3.3.3
Tween 20
concentration-dependent activation up to 152%
Rattus sp.
General Stability (protein specific)
EC Number
General Stability
Organism
1.3.3.3
purified enzyme rather unstable, 2 days
Rattus sp.
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.3.3.3
diethyl dicarbonate
concentration-dependent inhibition, complete inactivation at 50 mM
Rattus sp.
1.3.3.3
KCl
70% inhibition at 1 M
Rattus sp.
1.3.3.3
N-bromosuccinimide
concentration-dependent inhibition, complete inactivation at 50 mM
Rattus sp.
1.3.3.3
NaCl
60% inhibition at 1 M
Rattus sp.
1.3.3.3
Phenylglyoxal
concentration-dependent inhibition, complete inactivation at 50 mM
Rattus sp.
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.3.3.3
mitochondrion
located within intermembrane space
Rattus sp.
5739
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.3.3.3
38000
-
2 * 38000, SDS-PAGE
Rattus sp.
1.3.3.3
77000
-
minimum catalytic activity, also aggregation to 154000 kDa catalytic activity, gel filtration
Rattus sp.
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.3.3.3
coproporphyrinogen-III + O2
Rattus sp.
6th enzyme in heme biosynthetic pathway
protoporphyrinogen-IX + CO2
-
Rattus sp.
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.3.3.3
to homogeneity, chromatography techniques
Rattus sp.
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.3.3.3
liver
-
Rattus sp.
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.3.3.3
0.009
-
-
Rattus sp.
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.3.3.3
-20°C, 30-65% ammonium sulfate precipitate, 2 months
Rattus sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.3.3.3
coproporphyrinogen-III + O2
sixths enzyme in heme biosynthetic pathway
390943
Rattus sp.
protoporphyrinogen-IX + CO2
-
390943
Rattus sp.
?
1.3.3.3
coproporphyrinogen-III + O2
6th enzyme in heme biosynthetic pathway
390943
Rattus sp.
protoporphyrinogen-IX + CO2
-
390943
Rattus sp.
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.3.3.3
dimer
2 * 38000, SDS-PAGE
Rattus sp.