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Literature summary extracted from

  • Ullrich, A.; Knecht, W.; Fries, M.; Loffler, M.
    Recombinant expression of N-terminal truncated mutants of the membrane bound mouse, rat and human flavoenzyme dihydroorotate dehydrogenase. A versatile tool to rate inhibitor effects? (2001), Eur. J. Biochem., 268, 1861-1868.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.3.5.2 medicine great interest in inhibitors as potential therapeutic agents for the treatment of diseases involving aberrant cell proliferation Mus musculus
1.3.5.2 medicine great interest in inhibitors as potential therapeutic agents for the treatment of diseases involving aberrant cell proliferation Homo sapiens
1.3.5.2 medicine great interest in inhibitors as potential therapeutic agents for the treatment of diseases involving aberrant cell proliferation Rattus norvegicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.5.2 expressed in Escherichia coli XL-1 Blue, histidine tagged Mus musculus
1.3.5.2 expressed in Escherichia coli XL-1 Blue, histidine tagged Homo sapiens
1.3.5.2 expressed in Escherichia coli XL-1 Blue, histidine tagged Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.5.2 A77 1726 malononitrilamide, 50% inhibition at 18-773 nM Homo sapiens
1.3.5.2 A77 1726 malononitrilamide, 50% inhibition at 18-773 nM Mus musculus
1.3.5.2 A77 1726 malononitrilamide, 50% inhibition at 18-773 nM Rattus norvegicus
1.3.5.2 brequinar sodium 50% inhibition at 6-127 nM Homo sapiens
1.3.5.2 brequinar sodium 50% inhibition at 6-127 nM Mus musculus
1.3.5.2 brequinar sodium 50% inhibition at 6-127 nM Rattus norvegicus
1.3.5.2 MNA 279 malononitrilamide, 50% inhibition at 22-715 nM Homo sapiens
1.3.5.2 MNA 279 malononitrilamide, 50% inhibition at 22-715 nM Mus musculus
1.3.5.2 MNA 279 malononitrilamide, 50% inhibition at 22-715 nM Rattus norvegicus
1.3.5.2 MNA 715 malononitrilamide, 50% inhibition at 41-109 nM Homo sapiens
1.3.5.2 MNA 715 malononitrilamide, 50% inhibition at 41-109 nM Mus musculus
1.3.5.2 MNA 715 malononitrilamide, 50% inhibition at 41-109 nM Rattus norvegicus
1.3.5.2 redoxal 50% inhibition at 45-88 nM Homo sapiens
1.3.5.2 redoxal 50% inhibition at 45-88 nM Mus musculus
1.3.5.2 redoxal 50% inhibition at 45-88 nM Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.5.2 0.007
-
 ubiquinone
-
 Rattus norvegicus
1.3.5.2 0.01
-
 S-dihydroorotate
-
 Homo sapiens
1.3.5.2 0.011
-
 S-dihydroorotate
-
 Rattus norvegicus
1.3.5.2 0.014
-
 ubiquinone
-
 Homo sapiens
1.3.5.2 0.026
-
 S-dihydroorotate
-
 Mus musculus
1.3.5.2 0.062
-
 ubiquinone
-
 Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.5.2 mitochondrion inner membrane Mus musculus 5739
-
1.3.5.2 mitochondrion inner membrane Homo sapiens 5739
-
1.3.5.2 mitochondrion inner membrane Rattus norvegicus 5739
-
1.3.5.2 mitochondrion integral protein Homo sapiens 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.5.2 43000
-
 x * 43000, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.5.2 (S)-dihydroorotate + ubiquinone Mus musculus fourth enzyme in pyrimidine synthesis orotate + ubiquinol
-
 ?
1.3.5.2 (S)-dihydroorotate + ubiquinone Homo sapiens fourth enzyme in pyrimidine synthesis orotate + ubiquinol
-
 ?
1.3.5.2 (S)-dihydroorotate + ubiquinone Rattus norvegicus fourth enzyme in pyrimidine synthesis orotate + ubiquinol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.5.2 Homo sapiens
-
-
-
1.3.5.2 Mus musculus
-
-
-
1.3.5.2 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.5.2 Ni2+/nitrilotriacetate column Mus musculus
1.3.5.2 Ni2+/nitrilotriacetate column Homo sapiens
1.3.5.2 Ni2+/nitrilotriacetate column Rattus norvegicus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.5.2 101
-
-
 Mus musculus
1.3.5.2 107
-
-
 Homo sapiens
1.3.5.2 125
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
1.3.5.2 great inactivation by repeated thawing and freezing Mus musculus
1.3.5.2 great inactivation by repeated thawing and freezing Homo sapiens
1.3.5.2 great inactivation by repeated thawing and freezing Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.5.2 (S)-dihydroorotate + ubiquinone
-
 Mus musculus orotate + ubiquinol
-
 ?
1.3.5.2 (S)-dihydroorotate + ubiquinone
-
 Homo sapiens orotate + ubiquinol
-
 ?
1.3.5.2 (S)-dihydroorotate + ubiquinone
-
 Rattus norvegicus orotate + ubiquinol
-
 ?
1.3.5.2 (S)-dihydroorotate + ubiquinone fourth enzyme in pyrimidine synthesis Mus musculus orotate + ubiquinol
-
 ?
1.3.5.2 (S)-dihydroorotate + ubiquinone fourth enzyme in pyrimidine synthesis Homo sapiens orotate + ubiquinol
-
 ?
1.3.5.2 (S)-dihydroorotate + ubiquinone fourth enzyme in pyrimidine synthesis Rattus norvegicus orotate + ubiquinol
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.5.2 ?
-
 Rattus norvegicus
1.3.5.2 ? x * 43000, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
1.3.5.2 DHODH
-
 Mus musculus
1.3.5.2 DHODH
-
 Homo sapiens
1.3.5.2 DHODH
-
 Rattus norvegicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.5.2 71
-
 S-dihydroorotate
-
 Mus musculus
1.3.5.2 75
-
 S-dihydroorotate
-
 Homo sapiens
1.3.5.2 95
-
 S-dihydroorotate
-
 Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.5.2 8
-
-
 Homo sapiens
1.3.5.2 8
-
-
 Rattus norvegicus
1.3.5.2 8 8.1
-
 Mus musculus
1.3.5.2 8 8.1
-
 Homo sapiens
1.3.5.2 8 8.1
-
 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.5.2 additional information flavoenzyme, 0.6-1.2 mol flavin per mol protein Mus musculus
1.3.5.2 additional information flavoenzyme, 0.6-1.2 mol flavin per mol protein Homo sapiens
1.3.5.2 additional information flavoenzyme, 0.6-1.2 mol flavin per mol protein Rattus norvegicus