EC Number | Application | Comment | Organism |
---|---|---|---|
1.3.5.2 | medicine | molecular target of the antiproliferative, immunosuppressive compound brequinar sodium | Homo sapiens |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.3.5.2 | expressed in Escherichia coli DH5alpha TAP330, lacking the endogenous gene for the bacterial dihydroorotate dehydrogenase, pyrimidine auxotroph | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.3.5.2 | H11A | minimal effect on the relative enzyme activity | Homo sapiens |
1.3.5.2 | H122A | minimal effect on the relative enzyme activity | Homo sapiens |
1.3.5.2 | H129A | complete loss of enzymatic activity, conserved between the human and rat enzyme, required for enzymatic activity | Homo sapiens |
1.3.5.2 | H218A | minimal effect on the relative enzyme activity | Homo sapiens |
1.3.5.2 | H26A | minimal effect on the relative enzyme activity, insensitive against brequinar sodium inhibition, suggested location within the brequinar sodium binding pocket, important role in brequinar sodium binding to enzyme | Homo sapiens |
1.3.5.2 | H364A | complete loss of enzymatic activity, conserved between the human and rat enzyme, required for enzymatic activity | Homo sapiens |
1.3.5.2 | H56A | minimal effect on the relative enzyme activity | Homo sapiens |
1.3.5.2 | H71A | complete loss of enzymatic activity, surprising because no conserved residue in the closely related rat enzyme | Homo sapiens |
1.3.5.2 | H71N | comparable activity to wild-type, taken together with the results for H71A mutant, the histidine residue is not required at this position, but this site is less permissive than most of the other histidine locations within the enzyme | Homo sapiens |
1.3.5.2 | additional information | substitution of all histidine residues by alanine | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.3.5.2 | brequinar sodium | tight binding, most potent inhibitor, mutant H26A is insensitive, wild-type and other mutants are inhibited by 50% at concentrations between 6 and 10 nM | Homo sapiens | |
1.3.5.2 | diethyldicarbonate | histidine-selective covalent modifier | Homo sapiens | |
1.3.5.2 | leflunomide | - |
Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.5.2 | additional information | - |
additional information | relative Km-values for wild-type and mutants listed for (S)-dihydroorotate and ubiquinone-6 | Homo sapiens | |
1.3.5.2 | 0.0155 | - |
S-dihydroorotate | - |
Homo sapiens | |
1.3.5.2 | 0.028 | - |
ubiquinone-6 | - |
Homo sapiens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.3.5.2 | mitochondrion | - |
Homo sapiens | 5739 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.3.5.2 | 39000 | - |
- |
Homo sapiens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.5.2 | Homo sapiens | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.3.5.2 | liver | - |
Homo sapiens | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.5.2 | (S)-dihydroorotate + acceptor | ubiquinone-6 tested as electron acceptor | Homo sapiens | orotate + reduced acceptor | - |
? | |
1.3.5.2 | L-dihydroorotate + ubiquinone-6 | - |
Homo sapiens | orotate + reduced ubiquinone-6 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.3.5.2 | DHODase | - |
Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.3.5.2 | 23 | - |
assay at | Homo sapiens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.5.2 | additional information | - |
additional information | relative turnover numbers for wild-type and mutants listed for (S)-dihydroorotate and ubiquinone6 | Homo sapiens |