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Literature summary extracted from

  • Oba, K.; Ishikawa, S.; Nishikawa, M.; Mizuno, H.; Yamamoto, T.
    Purification and properties of L-galactono-gamma-lactone dehydrogenase, a key enzyme for ascorbic acid biosynthesis, from sweet potato roots (1995), J. Biochem., 117, 120-124.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.2.3 Acriflavine diminished by addition of FAD or FMN Ipomoea batatas
1.3.2.3 L-galactono-gamma-lactone substrate inhibition at concentration above 4.2 mM Ipomoea batatas
1.3.2.3 p-chloromercuribenzoate reduced by addition of 2-mercapthoethanol Ipomoea batatas

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.2.3 0.12
-
L-galactono-gamma-lactone
-
Ipomoea batatas

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.2.3 mitochondrion
-
Ipomoea batatas 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.2.3 56000
-
gel filtration Ipomoea batatas

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.2.3 L-galactono-1,4-lactone + 2 ferricytochrome c Ipomoea batatas terminal step in L-ascorbic acid biosynthesis L-ascorbate + 2 ferrocytochrome c + 2 H+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.3.2.3 Ipomoea batatas
-
sweet potato
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.2.3 low recovery Ipomoea batatas

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.2.3 root
-
Ipomoea batatas
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.2.3 37000
-
purified enzyme Ipomoea batatas

Storage Stability

EC Number Storage Stability Organism
1.3.2.3 -80°C, 0.3 mg per ml protein, 0.1 M potassium phosphate, pH 7.4, 10% glycerol,2 mM glutathione, 2 month Ipomoea batatas
1.3.2.3 -80°C, diluted protein, 0.1 M potassium phosphate, pH 7.4, 10% glycerol,2 mM glutathione, 30% loss of activity after 1 month Ipomoea batatas

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.2.3 L-galactono-1,4-lactone + 2 ferricytochrome c terminal step in L-ascorbic acid biosynthesis Ipomoea batatas L-ascorbate + 2 ferrocytochrome c + 2 H+
-
?
1.3.2.3 L-galactono-1,4-lactone + ferricytochrome c specific for L-galactono-gamma-lactone Ipomoea batatas L-ascorbate + ferrocytochrome c
-
?
1.3.2.3 L-gulono-gamma-lactone + ferricytochrome very low activity Ipomoea batatas D-ascorbate + ferricytochrome c
-
?

Subunits

EC Number Subunits Comment Organism
1.3.2.3 monomer 1 * 56000, SDS-PAGE Ipomoea batatas

Synonyms

EC Number Synonyms Comment Organism
1.3.2.3 GLDHase
-
Ipomoea batatas

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.2.3 27
-
assay at Ipomoea batatas

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.2.3 7.9
-
-
Ipomoea batatas

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.2.3 flavin probably Ipomoea batatas