BRENDA - Enzyme Database

Enoyl coenzyme A reduction by bovine mammary fatty acid synthetase. Specificity and other characteristics

Strom, K.A.; Galeos, W.L.; Davidson, L.A.; Kumar, S.; J. Biol. Chem. 254, 8153-8158 (1979)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
1.3.1.8
fatty acid synthetase with enoyl-CoA reductase activity: denaturation by mechanical agitation, exposure to air, uneven surfaces such as scratched glass, membranes for ultrafiltration
Bos taurus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.3.1.8
butyryl-CoA
-
Bos taurus
1.3.1.8
NADP+
-
Bos taurus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.3.1.8
Bos taurus
-
-
-
Oxidation Stability
EC Number
Oxidation Stability
Organism
1.3.1.8
denaturation by exposure to air
Bos taurus
Purification (Commentary)
EC Number
Commentary
Organism
1.3.1.8
fatty acid synthetase, also exhibiting crotonyl-CoA reductase activity
Bos taurus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.3.1.8
mammary gland
-
Bos taurus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.3.1.8
1
-
-
Bos taurus
Storage Stability
EC Number
Storage Stability
Organism
1.3.1.8
liquid N2, 30-50% loss of activity by thawing
Bos taurus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.3.1.8
2-methyl-trans-crotonyl-CoA + NADPH
2% of trans-crotonyl-CoA activity
390851
Bos taurus
2-methylbutanoyl-CoA + NADP+
-
-
-
?
1.3.1.8
2-trans-dodecenoyl-CoA + NAD(P)H
15% of trans-crotonyl-CoA activity
390851
Bos taurus
dodecanoyl-CoA + NAD(P)+
-
-
-
?
1.3.1.8
2-trans-hexadecenoyl-CoA + NADPH
13% of trans-crotonyl-CoA activity
390851
Bos taurus
hexadecanoyl CoA + NADP+
-
-
-
?
1.3.1.8
2-trans-hexenoyl-CoA + NAD(P)H
30% of trans-crotonyl-CoA activity
390851
Bos taurus
hexanoyl-CoA + NADP+
-
-
-
?
1.3.1.8
3-butenoyl-CoA + NADPH
7% of trans-crotonyl-CoA activity
390851
Bos taurus
butanoyl-CoA + NADP+
-
-
-
?
1.3.1.8
3-methyl-crotonyl-CoA + NADPH
17% of trans-crotonyl-CoA activity
390851
Bos taurus
3-methylbutanoyl-CoA + NADP+
-
-
-
?
1.3.1.8
crotonyl-CoA + NAD(P)H
cis-crotonyl-CoA 30% of trans-crotonyl-CoA activity
390851
Bos taurus
butanoyl-CoA + NADP+
-
-
-
?
1.3.1.8
additional information
no reduction of trans-crotonyl-S-pantetheine
390851
Bos taurus
?
-
-
-
-
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.3.1.8
NADPH
inactive with NADH
Bos taurus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.3.1.8
NADPH
inactive with NADH
Bos taurus
General Stability (protein specific)
EC Number
General Stability
Organism
1.3.1.8
fatty acid synthetase with enoyl-CoA reductase activity: denaturation by mechanical agitation, exposure to air, uneven surfaces such as scratched glass, membranes for ultrafiltration
Bos taurus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.3.1.8
butyryl-CoA
-
Bos taurus
1.3.1.8
NADP+
-
Bos taurus
Oxidation Stability (protein specific)
EC Number
Oxidation Stability
Organism
1.3.1.8
denaturation by exposure to air
Bos taurus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.3.1.8
fatty acid synthetase, also exhibiting crotonyl-CoA reductase activity
Bos taurus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.3.1.8
mammary gland
-
Bos taurus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.3.1.8
1
-
-
Bos taurus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.3.1.8
liquid N2, 30-50% loss of activity by thawing
Bos taurus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.3.1.8
2-methyl-trans-crotonyl-CoA + NADPH
2% of trans-crotonyl-CoA activity
390851
Bos taurus
2-methylbutanoyl-CoA + NADP+
-
-
-
?
1.3.1.8
2-trans-dodecenoyl-CoA + NAD(P)H
15% of trans-crotonyl-CoA activity
390851
Bos taurus
dodecanoyl-CoA + NAD(P)+
-
-
-
?
1.3.1.8
2-trans-hexadecenoyl-CoA + NADPH
13% of trans-crotonyl-CoA activity
390851
Bos taurus
hexadecanoyl CoA + NADP+
-
-
-
?
1.3.1.8
2-trans-hexenoyl-CoA + NAD(P)H
30% of trans-crotonyl-CoA activity
390851
Bos taurus
hexanoyl-CoA + NADP+
-
-
-
?
1.3.1.8
3-butenoyl-CoA + NADPH
7% of trans-crotonyl-CoA activity
390851
Bos taurus
butanoyl-CoA + NADP+
-
-
-
?
1.3.1.8
3-methyl-crotonyl-CoA + NADPH
17% of trans-crotonyl-CoA activity
390851
Bos taurus
3-methylbutanoyl-CoA + NADP+
-
-
-
?
1.3.1.8
crotonyl-CoA + NAD(P)H
cis-crotonyl-CoA 30% of trans-crotonyl-CoA activity
390851
Bos taurus
butanoyl-CoA + NADP+
-
-
-
?
1.3.1.8
additional information
no reduction of trans-crotonyl-S-pantetheine
390851
Bos taurus
?
-
-
-
-