EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.3.1.8 | coenzyme A | free form, strong activator of the reduction | Bos taurus | |
1.3.1.86 | CoA | free CoA is a strong activator of the reductase reaction | Bos taurus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.3.1.8 | 2-methylcrotonyl-CoA | CoA-activated enzyme, competitive inhibition | Bos taurus | |
1.3.1.8 | 3-methylcrotonyl-CoA | - |
Bos taurus | |
1.3.1.8 | 4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole | - |
Bos taurus | |
1.3.1.8 | 5,5'-dithiobis(2-nitrobenzoate) | - |
Bos taurus | |
1.3.1.8 | acetyl-CoA | competitive | Bos taurus | |
1.3.1.8 | butyryl-CoA | competitive | Bos taurus | |
1.3.1.8 | coenzyme A | in free form inhibits fatty acid sythesis | Bos taurus | |
1.3.1.8 | malonyl-CoA | non-competitive | Bos taurus | |
1.3.1.8 | methylmalonyl-CoA | i.e. tiglyl-CoA | Bos taurus | |
1.3.1.86 | 2-methylcrotonyl-CoA | CoA-activated enzyme shows competitive inhibition with the substrate analog 2-methylcrotonyl-CoA | Bos taurus | |
1.3.1.86 | 4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole | extremely potent inhibitor | Bos taurus | |
1.3.1.86 | 5,5'-dithiobis(2-nitrobenzoic acid) | extremely potent inhibitor | Bos taurus | |
1.3.1.86 | acetyl-CoA | competitive inhibitor | Bos taurus | |
1.3.1.86 | butyryl-CoA | competitive inhibitor | Bos taurus | |
1.3.1.86 | iodoacetamide | - |
Bos taurus | |
1.3.1.86 | iodoacetate | - |
Bos taurus | |
1.3.1.86 | malonyl-CoA | noncompetitive inhibitor | Bos taurus | |
1.3.1.86 | N-ethylmaleimide | extremely potent inhibitor | Bos taurus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.1.8 | Bos taurus | - |
- |
- |
1.3.1.86 | Bos taurus | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.3.1.8 | mammary gland | - |
Bos taurus | - |
1.3.1.86 | mammary gland | - |
Bos taurus | - |
EC Number | Storage Stability | Organism |
---|---|---|
1.3.1.8 | 2-3°C, absence of dithiothreitol, half life 3 h | Bos taurus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.1.8 | crotonyl-CoA + NAD(P)H | - |
Bos taurus | butanoyl-CoA + NADP+ | - |
? |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.3.1.8 | 8 | - |
above pH 8.0, effect of CoA on pH-profile | Bos taurus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.3.1.8 | 6 | 8.3 | with CoA changed from sigmoidal curve to flat one | Bos taurus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.3.1.8 | NADPH | inactive with NADH | Bos taurus |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.1.8 | 0.0029 | - |
2-methylcrotonyl-CoA | - |
Bos taurus | |
1.3.1.8 | 0.005 | - |
butyryl-CoA | - |
Bos taurus | |
1.3.1.8 | 0.006 | - |
acetyl-CoA | - |
Bos taurus | |
1.3.1.8 | 0.021 | - |
malonyl-CoA | - |
Bos taurus | |
1.3.1.86 | 0.0029 | - |
2-methylcrotonyl-CoA | pH and temperature not specified in the publication | Bos taurus | |
1.3.1.86 | 0.005 | - |
butyryl-CoA | pH and temperature not specified in the publication | Bos taurus | |
1.3.1.86 | 0.006 | - |
acetyl-CoA | pH and temperature not specified in the publication | Bos taurus | |
1.3.1.86 | 0.021 | - |
malonyl-CoA | pH and temperature not specified in the publication | Bos taurus |