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Literature summary extracted from

  • Strom, K.A.; Kumar, S.
    Activation and inhibition of crotonyl-coenzyme A reductase activity of bovine mammary fatty acid synthetase (1979), J. Biol. Chem., 254, 8159-8162.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.3.1.8 coenzyme A free form, strong activator of the reduction Bos taurus
1.3.1.86 CoA free CoA is a strong activator of the reductase reaction Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.8 2-methylcrotonyl-CoA CoA-activated enzyme, competitive inhibition Bos taurus
1.3.1.8 3-methylcrotonyl-CoA
-
Bos taurus
1.3.1.8 4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole
-
Bos taurus
1.3.1.8 5,5'-dithiobis(2-nitrobenzoate)
-
Bos taurus
1.3.1.8 acetyl-CoA competitive Bos taurus
1.3.1.8 butyryl-CoA competitive Bos taurus
1.3.1.8 coenzyme A in free form inhibits fatty acid sythesis Bos taurus
1.3.1.8 malonyl-CoA non-competitive Bos taurus
1.3.1.8 methylmalonyl-CoA i.e. tiglyl-CoA Bos taurus
1.3.1.86 2-methylcrotonyl-CoA CoA-activated enzyme shows competitive inhibition with the substrate analog 2-methylcrotonyl-CoA Bos taurus
1.3.1.86 4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole extremely potent inhibitor Bos taurus
1.3.1.86 5,5'-dithiobis(2-nitrobenzoic acid) extremely potent inhibitor Bos taurus
1.3.1.86 acetyl-CoA competitive inhibitor Bos taurus
1.3.1.86 butyryl-CoA competitive inhibitor Bos taurus
1.3.1.86 iodoacetamide
-
Bos taurus
1.3.1.86 iodoacetate
-
Bos taurus
1.3.1.86 malonyl-CoA noncompetitive inhibitor Bos taurus
1.3.1.86 N-ethylmaleimide extremely potent inhibitor Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.8 Bos taurus
-
-
-
1.3.1.86 Bos taurus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.1.8 mammary gland
-
Bos taurus
-
1.3.1.86 mammary gland
-
Bos taurus
-

Storage Stability

EC Number Storage Stability Organism
1.3.1.8 2-3°C, absence of dithiothreitol, half life 3 h Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.8 crotonyl-CoA + NAD(P)H
-
Bos taurus butanoyl-CoA + NADP+
-
?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.8 8
-
above pH 8.0, effect of CoA on pH-profile Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.3.1.8 6 8.3 with CoA changed from sigmoidal curve to flat one Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.8 NADPH inactive with NADH Bos taurus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.3.1.8 0.0029
-
2-methylcrotonyl-CoA
-
Bos taurus
1.3.1.8 0.005
-
butyryl-CoA
-
Bos taurus
1.3.1.8 0.006
-
acetyl-CoA
-
Bos taurus
1.3.1.8 0.021
-
malonyl-CoA
-
Bos taurus
1.3.1.86 0.0029
-
2-methylcrotonyl-CoA pH and temperature not specified in the publication Bos taurus
1.3.1.86 0.005
-
butyryl-CoA pH and temperature not specified in the publication Bos taurus
1.3.1.86 0.006
-
acetyl-CoA pH and temperature not specified in the publication Bos taurus
1.3.1.86 0.021
-
malonyl-CoA pH and temperature not specified in the publication Bos taurus