Literature summary extracted from

Zweytick, D.; Hrastnik, C.; Kohlwein, S.D.; Daum, G.
Biochemical characterization and subcellular localization of the sterol C-24(28) reductase, Erg4p, from the yeast Saccharomyces cerevisiae (2000), FEBS Lett., 470, 83-87.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
1.3.1.71	green fluorescence protein fusion protein expressed in Saccharomyces cerevisiae erg4 mutant	Saccharomyces cerevisiae

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
1.3.1.71	endoplasmic reticulum	-	Saccharomyces cerevisiae	5783	-

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
1.3.1.71	56000	-	x * 56000, deduced from immunoblot	Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
1.3.1.71	ergosta-5,7,22,24(241)-tetraen-3beta-ol + NADPH + H+	Saccharomyces cerevisiae	-	ergosta-5,7,22-trien-3beta-ol + NADP+	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.3.1.71	Saccharomyces cerevisiae	-	-	-

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.3.1.71	0.00032	-	enzyme activity in 40000 x g microsomal fraction, corresponding to endoplasmic reticulum membranes	Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.3.1.71	ergosta-5,7,22,24(241)-tetraen-3beta-ol + NADPH + H+	-	Saccharomyces cerevisiae	ergosta-5,7,22-trien-3beta-ol + NADP+	-	?
1.3.1.71	ergosta-5,7,22-trien-3beta-ol + NADP+	-	Saccharomyces cerevisiae	ergosta-5,7,22,24(241)-tetraen-3beta-ol + NADPH + H+	-	?

Subunits

EC Number	Subunits	Comment	Organism
1.3.1.71	?	x * 56000, deduced from immunoblot	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)