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Literature summary extracted from

  • Schabort, J.C.; Potgieter, D.J.J.; De Villiers, V.
    Cucurbitacin B DELTA23-reductase from Cucurbita maxima. I. Assay methods, isolation and purification (1968), Biochim. Biophys. Acta, 151, 33-46.
    View publication on PubMed

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.5 Cucurbita maxima
-
 var. Green Hubbard
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.5
-
 Cucurbita maxima

Reaction

EC Number Reaction Comment Organism Reaction ID
1.3.1.5 23,24-dihydrocucurbitacin B + NAD(P)+ = cucurbitacin B + NAD(P)H + H+ plant terpenoid Cucurbita maxima
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.1.5 fruit
-
 Cucurbita maxima
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.5 5812
-
-
 Cucurbita maxima

Storage Stability

EC Number Storage Stability Organism
1.3.1.5 0-4°C, maleic acid-NaOH buffer, pH 6.6, 100 mM NaCl, 5 weeks Cucurbita maxima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.5 cucurbitacin B + NAD(P)H + H+
-
 Cucurbita maxima 23,24-dihydrocucurbitacin B + NAD(P)+
-
 ir

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.5 25
-
 assay at Cucurbita maxima

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.3.1.5 50
-
 5 min, no loss of activity, 10 min, 30% loss of activity Cucurbita maxima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.5 6.65
-
 crude enzyme preparation in presence of Zn2+ has a wider pH optimum range than purified enzyme in presence of Mn2+ Cucurbita maxima

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.3.1.5 5 8 pH 5.0: about 25% of activity at maximum, pH 8.0: about 80% of activity at maximum of crude enzyme preparation in presence of Zn2+, about 10% of activity at maximum of purified enzyme in presence of Mn2+ Cucurbita maxima

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.5 NAD(P)+
-
 Cucurbita maxima