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Literature summary extracted from

  • Hansen, H.S.
    Purification and characterization of a 15-ketoprostaglandin delta 13-reductase from bovine lung (1979), Biochim. Biophys. Acta, 574, 136-145.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.48 NADP+
-
 Bos taurus
1.3.1.48 p-chloromercuribenzoate 0.1 mM, complete inhibition Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.48 0.005 0.009 NADPH
-
 Bos taurus
1.3.1.48 0.088 0.094 NADH
-
 Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.48 56000
-
 gel filtration Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.48 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.48
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.1.48 lung
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.48 1.067
-
-
 Bos taurus

Storage Stability

EC Number Storage Stability Organism
1.3.1.48 -4°C, 0.1 M sodium phosphate, pH 7.4, 1 mM EDTA, 1 mM mercaptoethanol, stable for more than 1 month Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.48 (5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NAD(P)H no substrate: prostaglandin E1 Bos taurus (5Z)-11alpha-hydroxy-9,15-dioxoprost-5-enoate + NAD(P)+
-
 ir
1.3.1.48 15-ketoprostaglandin E + NAD(P)H
-
 Bos taurus ?
-
 ?
1.3.1.48 15-ketoprostaglandin E2 + NADH + H+
-
 Bos taurus 13,14-dihydro-15-ketoprostaglandin E2 + NAD+
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.48 37
-
 assay at Bos taurus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.1.48 0.7 1 15-ketoprostaglandin E
-
 Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.48 NAD(P)H bovine lung: enzyme can use both NADPH and NADH Bos taurus