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Literature summary extracted from

  • Nishimaki, T.; Yamanaka, H.; Mizugaki, M.
    Studies on the metabolism of unsaturated fatty acids. XIV. Purification and properties of NADPH-dependent trans-2-enoyl-CoA reductase of Escherichia coli K-12 (1984), J. Biochem., 95, 1315-1321.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.3.1.38 additional information no flavoprotein Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.38 5,5'-dithiobis(2-nitrobenzoic acid) i.e. DTNB, inhibition can be prevented by preincubation with NADPH, thus a SH-group is involved in cofactor binding Escherichia coli
1.3.1.38 bovine serum albumin
-
 Escherichia coli
1.3.1.38 decanoyl-CoA
-
 Escherichia coli
1.3.1.38 lauroyl-CoA
-
 Escherichia coli
1.3.1.38 additional information not inhibitory: methylglyoxal Escherichia coli
1.3.1.38 myristoyl-CoA
-
 Escherichia coli
1.3.1.38 N-ethylmaleimide
-
 Escherichia coli
1.3.1.38 NADP+
-
 Escherichia coli
1.3.1.38 octanoyl-CoA
-
 Escherichia coli
1.3.1.38 p-hydroxymercuribenzoate
-
 Escherichia coli
1.3.1.38 palmitoleoyl-CoA
-
 Escherichia coli
1.3.1.38 palmitoyl-CoA
-
 Escherichia coli
1.3.1.38 Saturated CoA analogs
-
 Escherichia coli
1.3.1.38 Triton X-100
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.38 0.0037
-
 trans-2-dodecenoyl-CoA
-
 Escherichia coli
1.3.1.38 0.0049
-
 NADPH
-
 Escherichia coli
1.3.1.38 0.0071
-
 trans-2-decenoyl-CoA
-
 Escherichia coli
1.3.1.38 0.011
-
 trans-2-octenoyl-CoA
-
 Escherichia coli
1.3.1.38 0.048
-
 trans-2-hexenoyl-CoA
-
 Escherichia coli
1.3.1.38 0.072
-
 trans-2-crotonyl-CoA
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.38 40000
-
 2 * 40000, SDS-PAGE Escherichia coli
1.3.1.38 80000
-
 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.38 trans-2-hexenoyl-CoA + NADPH + H+ Escherichia coli
-
 hexanoyl-CoA + NADP+
-
 ?
1.3.1.38 trans-2-octenoyl-CoA + NADPH + H+ Escherichia coli
-
 octanoyl-CoA + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.38 Escherichia coli
-
 K12
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.38
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.38 20
-
 purified enzyme Escherichia coli

Storage Stability

EC Number Storage Stability Organism
1.3.1.38 -20°C, Tris-HCl buffer, pH 8.0, 10% v/v glycerol Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.38 crotonyl-CoA + NADPH
-
 Escherichia coli butanoyl-CoA + NADP+
-
 ?
1.3.1.38 additional information activity decreases with increasing chain length of substrates Escherichia coli ?
-
 ?
1.3.1.38 trans-2-decenoyl-CoA + NADPH
-
 Escherichia coli decanoyl-CoA + NADP+
-
 ?
1.3.1.38 trans-2-dodecenoyl-CoA
-
 Escherichia coli ?
-
 r
1.3.1.38 trans-2-hexenoyl-CoA + NADPH
-
 Escherichia coli hexanoyl-CoA + NADP+
-
 ?
1.3.1.38 trans-2-hexenoyl-CoA + NADPH + H+
-
 Escherichia coli hexanoyl-CoA + NADP+
-
 ?
1.3.1.38 trans-2-octenoyl-CoA + NADPH
-
 Escherichia coli octanoyl-CoA + NADP+
-
 ?
1.3.1.38 trans-2-octenoyl-CoA + NADPH + H+
-
 Escherichia coli octanoyl-CoA + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.38 dimer 2 * 40000, SDS-PAGE Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.38 NADPH specific for Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.3.1.38 0.00054
-
 palmitoleoyl-CoA
-
 Escherichia coli
1.3.1.38 0.0025
-
 palmitoyl-CoA
-
 Escherichia coli
1.3.1.38 0.0092
-
 myristoyl-CoA
-
 Escherichia coli
1.3.1.38 0.045
-
 lauroyl-CoA
-
 Escherichia coli
1.3.1.38 0.063
-
 decanoyl-CoA
-
 Escherichia coli
1.3.1.38 0.198
-
 octanoyl-CoA
-
 Escherichia coli