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Literature summary extracted from
- Studies on the metabolism of unsaturated fatty acids. II. Separation and general properties of reduced nicotinamide adenine dinucleotide phosphate dependent cis-2-enoyl-coenzyme A reductase from Escherichia coli K-12 (1979), Chem. Pharm. Bull., 27, 2334-2337.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.3.1.37 | iodoacetic acid | 1 mM, 34% inhibition | Escherichia coli |  |
| 1.3.1.37 | p-hydroxymercuribenzoate | 1 mM, 77% inhibition | Escherichia coli | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.3.1.37 | 0.02 | - |
cis-2-octenoyl-CoA | - |
Escherichia coli | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.3.1.37 | Escherichia coli | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.3.1.37 | - |
Escherichia coli |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.3.1.37 | 0.0005 | - |
- |
Escherichia coli |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.3.1.37 | -20°C, ammonium sulfate precipitate | Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.3.1.37 | cis-2-decenoyl-CoA + NADPH + H+ | no reduction of cis-3-enoyl substrates | Escherichia coli | decanoyl-CoA + NADP+ | - |
? | |
| 1.3.1.37 | cis-2-dodecenoyl-CoA + NADPH + H+ | - |
Escherichia coli | dodecanoyl-CoA + NADP+ | - |
? | |
| 1.3.1.37 | cis-2-octenoyl-CoA + NADPH + H+ | - |
Escherichia coli | octanoyl-CoA + NADP+ | - |
? | |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.3.1.37 | 45 | - |
no loss of activity after 30 min | Escherichia coli |
| 1.3.1.37 | 55 | - |
10 min, inactivation | Escherichia coli |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.3.1.37 | NADPH | - |
Escherichia coli | |
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Release 2023.1 (January 2023)
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