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Literature summary extracted from

  • Travkin, V.M.; Linko, E.V.; Golovleva, L.A.
    Purification and characterization of maleylacetate reductase from Nocardioides simplex 3E utilizing phenoxyalcanoic herbicides 2,4-D and 2,4,5-T (1999), Biochemistry (Moscow), 64, 625-630.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.3.1.32 enzyme is very unstable Pimelobacter simplex

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.32 0.025
-
 maleylacetate
-
 Pimelobacter simplex

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.32 37000
-
 2 * 37000, homodimer Pimelobacter simplex

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.32 2-maleylacetate + NADPH Pimelobacter simplex
-
 3-oxoadipate + NADP+
-
 ir
1.3.1.32 2-maleylacetate + NADPH Pimelobacter simplex 3E
-
 3-oxoadipate + NADP+
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.32 Pimelobacter simplex
-
 3E
-
1.3.1.32 Pimelobacter simplex 3E
-
 3E
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.32
-
 Pimelobacter simplex

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.32 2-maleylacetate + NAD(P)H
-
 Pimelobacter simplex 3-oxoadipate + NAD(P)+
-
 ir
1.3.1.32 2-maleylacetate + NAD(P)H
-
 Pimelobacter simplex 3E 3-oxoadipate + NAD(P)+
-
 ir
1.3.1.32 2-maleylacetate + NADPH
-
 Pimelobacter simplex 3-oxoadipate + NADP+
-
 ir
1.3.1.32 2-maleylacetate + NADPH
-
 Pimelobacter simplex 3E 3-oxoadipate + NADP+
-
 ir
1.3.1.32 phenoxyalacanoic herbicide 2,4,5-T + NADH
-
 Pimelobacter simplex ? + NAD+
-
 ?
1.3.1.32 phenoxyalacanoic herbicide 2,4,5-T + NADH
-
 Pimelobacter simplex 3E ? + NAD+
-
 ?
1.3.1.32 phenoxyalacanoic herbicide 2,4-D + NADH
-
 Pimelobacter simplex ? + NAD+
-
 ?
1.3.1.32 phenoxyalacanoic herbicide 2,4-D + NADH
-
 Pimelobacter simplex 3E ? + NAD+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.32 dimer 2 * 37000, homodimer Pimelobacter simplex

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.32 50
-
-
 Pimelobacter simplex

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.1.32 114
-
 maleylacetate
-
 Pimelobacter simplex

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.32 7 7.1
-
 Pimelobacter simplex