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Literature summary extracted from

  • Daubaras, D.L.; Saido, K.; Chakrabarty, A.M.
    Purification of hydroxyquinol 1,2-dioxygenase and maleylacetate reductase: the lower pathway of 2,4,5-trichlorophenoxyacetic acid metabolism by Burkholderia cepacia AC1100 (1996), Appl. Environ. Microbiol., 62, 4276-4279.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.32 gen tftE cloned and overexpressed in Escherichia coli Burkholderia cepacia
1.13.11.37 expressed in Escherichia coli Burkholderia cepacia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.37 0.007
-
 Hydroxyquinol
-
 Burkholderia cepacia

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.37 36000
-
 2 * 36000, SDS-PAGE Burkholderia cepacia
1.13.11.37 68000
-
 gel filtration Burkholderia cepacia

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.32 2-maleylacetate + NADPH Pseudomonas sp.
-
 3-oxoadipate + NADP+
-
 ir
1.3.1.32 2-maleylacetate + NADPH Burkholderia cepacia
-
 3-oxoadipate + NADP+
-
 ir
1.3.1.32 2-maleylacetate + NADPH Pseudomonas sp. B13
-
 3-oxoadipate + NADP+
-
 ir
1.3.1.32 2-maleylacetate + NADPH Burkholderia cepacia AC1100
-
 3-oxoadipate + NADP+
-
 ir
1.13.11.37 benzene-1,2,4-triol + O2 Burkholderia cepacia
-
 3-hydroxy-cis-cis-muconate
-
 ?
1.13.11.37 benzene-1,2,4-triol + O2 Burkholderia cepacia AC1100
-
 3-hydroxy-cis-cis-muconate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.32 Burkholderia cepacia
-
 AC1100
-
1.3.1.32 Burkholderia cepacia AC1100
-
 AC1100
-
1.3.1.32 Pseudomonas sp.
-
-
-
1.3.1.32 Pseudomonas sp. B13
-
-
-
1.13.11.37 Burkholderia cepacia
-
 AC1100, grown with 2,4,6-trichlorophenol
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.32 purified from recombinant Escherichia coli cells Burkholderia cepacia
1.13.11.37
-
 Burkholderia cepacia

Storage Stability

EC Number Storage Stability Organism
1.13.11.37 4°C, stable for 2 to 3 weeks in elution buffer, can not be stored at 4°C or frozen at -20°C Burkholderia cepacia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.32 2-maleylacetate + NAD(P)H
-
 Pseudomonas sp. 3-oxoadipate + NAD(P)+
-
 ir
1.3.1.32 2-maleylacetate + NAD(P)H
-
 Burkholderia cepacia 3-oxoadipate + NAD(P)+
-
 ir
1.3.1.32 2-maleylacetate + NAD(P)H
-
 Pseudomonas sp. B13 3-oxoadipate + NAD(P)+
-
 ir
1.3.1.32 2-maleylacetate + NAD(P)H
-
 Burkholderia cepacia AC1100 3-oxoadipate + NAD(P)+
-
 ir
1.3.1.32 2-maleylacetate + NADPH
-
 Pseudomonas sp. 3-oxoadipate + NADP+
-
 ir
1.3.1.32 2-maleylacetate + NADPH
-
 Burkholderia cepacia 3-oxoadipate + NADP+
-
 ir
1.3.1.32 2-maleylacetate + NADPH
-
 Pseudomonas sp. B13 3-oxoadipate + NADP+
-
 ir
1.3.1.32 2-maleylacetate + NADPH
-
 Burkholderia cepacia AC1100 3-oxoadipate + NADP+
-
 ir
1.13.11.37 benzene-1,2,4-triol + O2
-
 Burkholderia cepacia maleylacetate
-
 ?
1.13.11.37 benzene-1,2,4-triol + O2
-
 Burkholderia cepacia AC1100 maleylacetate
-
 ?
1.13.11.37 benzene-1,2,4-triol + O2
-
 Burkholderia cepacia 3-hydroxy-cis-cis-muconate
-
 ?
1.13.11.37 benzene-1,2,4-triol + O2
-
 Burkholderia cepacia AC1100 3-hydroxy-cis-cis-muconate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.37 dimer 2 * 36000, SDS-PAGE Burkholderia cepacia

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.37 37
-
-
 Burkholderia cepacia

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.13.11.37 23 52
-
 Burkholderia cepacia

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.37 6.35
-
 Hydroxyquinol
-
 Burkholderia cepacia

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.37 6.4
-
-
 Burkholderia cepacia

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.13.11.37 6 7.2
-
 Burkholderia cepacia

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.32 NADH
-
 Pseudomonas sp.
1.3.1.32 NADH
-
 Burkholderia cepacia