Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Thanos, I.; Bader, J.; Guenther, H.; Neumann, S.; Krauss, F.; Simon, H.
    Electroenzymatic and electromicrobial reduction: preparation of chiral compounds (1987), Methods Enzymol., 136, 302-317.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.3.1.31 enzyme immobilized in calcium alginate gel, used in operation for 2 weeks, half-life of about 200 h Clostridium tyrobutyricum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.31 Aliphatic carboxylates
-
 Clostridium tyrobutyricum
1.3.1.31 methyl viologen
-
 Clostridium tyrobutyricum
1.3.1.31 NAD+
-
 Clostridium tyrobutyricum
1.3.1.31 phenyl propionate
-
 Clostridium tyrobutyricum
1.3.1.31 reduced methyl viologen
-
 Clostridium tyrobutyricum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.31 0.012
-
 NADH
-
 Clostridium tyrobutyricum
1.3.1.31 0.013
-
 cinnamate
-
 Clostridium tyrobutyricum
1.3.1.31 0.014
-
 (E)-geraniate
-
 Clostridium tyrobutyricum
1.3.1.31 0.1
-
 (Z)-geraniate
-
 Clostridium tyrobutyricum
1.3.1.31 0.4
-
 reduced methyl viologen
-
 Clostridium tyrobutyricum
1.3.1.31 0.5
-
 (Z)-2-bromocinnamate
-
 Clostridium tyrobutyricum
1.3.1.31 1.5
-
 butenoate
-
 Clostridium tyrobutyricum

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.31 Clostridium tyrobutyricum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.31
-
 Clostridium tyrobutyricum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.31 20
-
 (E)-2-methylbutenoate Clostridium tyrobutyricum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.31 (E)-2-methyl-2-butenoate + NADH
-
 Clostridium tyrobutyricum 2-methylbutanoate + NAD+
-
 ?
1.3.1.31 (E)-2-methylbutenoate + NADH
-
 Clostridium tyrobutyricum (R)-2-methylbutanoate + NAD+
-
 ?
1.3.1.31 (E)-2-methylbutenoate + reduced methylviologen
-
 Clostridium tyrobutyricum (R)-2-methylbutanoate + methyl viologen
-
 ?
1.3.1.31 (E)-geraniate + NADH
-
 Clostridium tyrobutyricum ?
-
 ?
1.3.1.31 (Z)-2-bromocinnamate + NADH
-
 Clostridium tyrobutyricum 3-(2-bromophenyl)propionate + NAD+
-
 ?
1.3.1.31 (Z)-geraniate + NADH
-
 Clostridium tyrobutyricum ?
-
 ?
1.3.1.31 butenoate + NADH
-
 Clostridium tyrobutyricum butanoate + NAD+
-
 ?
1.3.1.31 butenoate + reduced methyl viologen
-
 Clostridium tyrobutyricum butanoate + methyl viologen
-
 ?
1.3.1.31 cinnamate + NADH
-
 Clostridium tyrobutyricum phenylpropionate + NAD+
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.31 5.2
-
-
 Clostridium tyrobutyricum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.3.1.31 4.5 6.2 about 65% of maximal activity at pH 4.5 and at pH 6.2 Clostridium tyrobutyricum

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.31 NADH
-
 Clostridium tyrobutyricum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.3.1.31 0.84
-
 NAD+
-
 Clostridium tyrobutyricum
1.3.1.31 10
-
 reduced methyl viologen
-
 Clostridium tyrobutyricum
1.3.1.31 20
-
 methyl viologen
-
 Clostridium tyrobutyricum
1.3.1.31 30
-
 phenyl propionate
-
 Clostridium tyrobutyricum