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Literature summary extracted from

  • Bertics, P.J.; Karavolas, H.J.
    Pituitary progesterone 5alpha-reductase: solubilization and partial characterization (1985), J. Steroid Biochem., 22, 795-802.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.3.1.22 dithiothreitol no influence on microsomal enzyme, but slightly stimulating the solubilized enzyme Rattus norvegicus
1.3.1.22 Phospholipids stimulate Rattus norvegicus

General Stability

EC Number General Stability Organism
1.3.1.22 dialysis at 4°C, 22 h, n-octylglycoside-KCl extracted enzyme without loss of activity Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.22 EDTA slightly inhibitory Rattus norvegicus
1.3.1.22 flavins and flavin analogs, high concentration Rattus norvegicus
1.3.1.22 p-chloromercuribenzoate complete inhibition Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.22 0.12
-
 progesterone
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.1.22 microsome
-
 Rattus norvegicus
-
-

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.22 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.1.22 hypophysis anterior Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.22 additional information
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.22 progesterone + NADPH + H+
-
 Rattus norvegicus 5alpha-pregnan-3-20-dione + NADP+
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.22 NADPH
-
 Rattus norvegicus