Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Tomkins, G.M.
    The enzymatic reduction of DELTA4-3-ketosteroids (1957), J. Biol. Chem., 225, 13-24.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.3.1.3 bovine serum albumin stabilizes Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.3 p-Chloromercuribenzyl sulfonate 2 mM, complete inhibition, 50% inhibition in the presence of 10 mM glutathione Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.3 0.014
-
 NADPH
-
 Rattus norvegicus
1.3.1.3 0.034
-
 cortisone
-
 Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.3 Bos taurus
-
-
-
1.3.1.3 Cavia porcellus
-
-
-
1.3.1.3 Columba sp.
-
-
-
1.3.1.3 Oryctolagus cuniculus
-
-
-
1.3.1.3 Rattus norvegicus
-
-
-
1.3.1.3 Sus scrofa
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.1.3 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.3 additional information
-
 105 units/mg, 1 unit is defined as a change in optical density of 0.001 in 20 min Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
1.3.1.3 -10°C, stable for 2-3 days Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.3 cortisone + NADPH + H+
-
 Cavia porcellus 5beta-17,21dihydroxy-pregnan-3,11,20-trione + NADP+
-
 ?
1.3.1.3 cortisone + NADPH + H+
-
 Sus scrofa 5beta-17,21dihydroxy-pregnan-3,11,20-trione + NADP+
-
 ?
1.3.1.3 cortisone + NADPH + H+
-
 Bos taurus 5beta-17,21dihydroxy-pregnan-3,11,20-trione + NADP+
-
 ?
1.3.1.3 cortisone + NADPH + H+
-
 Oryctolagus cuniculus 5beta-17,21dihydroxy-pregnan-3,11,20-trione + NADP+
-
 ?
1.3.1.3 cortisone + NADPH + H+
-
 Columba sp. 5beta-17,21dihydroxy-pregnan-3,11,20-trione + NADP+
-
 ?
1.3.1.3 cortisone + NADPH + H+ high substrate specificity Rattus norvegicus 5beta-17,21dihydroxy-pregnan-3,11,20-trione + NADP+
-
 ir

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.3.1.3 3
-
 complete loss of activity after 24 h Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.3 5.8
-
-
 Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.3.1.3 5.2 8 about 50% of activity maximum at pH 5.2 and 8 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.3 NADPH absolutely required Cavia porcellus
1.3.1.3 NADPH absolutely required Rattus norvegicus