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Literature summary extracted from

  • Sakaitani, M.; Rusnak, F.; Quinn, N.R.; Tu, C.; Frigo, R.B.; Berchtold, G.A.; Walsh, C.T.
    Mechanistic studies on trans-2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase (Ent A) in the biosynthesis of the iron chelator enterobactin (1990), Biochemistry, 29, 6789-6798.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.28 cis-2-hydroxy-3-cyclohexen-1-carboxylate
-
 Escherichia coli
1.3.1.28 cis-2-hydroxycyclohexane-1-carboxylate
-
 Escherichia coli
1.3.1.28 trans-2-hydroxycyclohexane-1-carboxylate
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.28 0.26
-
 methyl 2,3-dihydro-2,3-dihydroxybenzoate
-
 Escherichia coli
1.3.1.28 0.3
-
 (2S,3S)-2,3-dihydro-2,3-dihydroxybenzoate
-
 Escherichia coli
1.3.1.28 1.7
-
 methyl-3-hydroxy-1,4-cyclohexadiene-1-carboxylate
-
 Escherichia coli
1.3.1.28 1.9
-
 trans-3-hydroxy-2-cyclohexene-1-carboxylate
-
 Escherichia coli
1.3.1.28 2.8
-
 cis-3-hydroxy-4-cyclohexene-1-carboxylate
-
 Escherichia coli
1.3.1.28 4.1
-
 cis-3-hydroxycyclohexane-1-carboxylic acid
-
 Escherichia coli
1.3.1.28 16.5
-
 trans-3,5-cyclohexadiene-1,2-diol
-
 Escherichia coli
1.3.1.28 25.2
-
 trans-3-hydroxycyclohexane-1-carboxylic acid
-
 Escherichia coli
1.3.1.28 83.3
-
 2-Cyclohexen-1-ol
-
 Escherichia coli
1.3.1.28 168
-
 cis-3,5-cyclohexadiene-1,2-diol
-
 Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.28 Escherichia coli
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.3.1.28 (2S,3S)-2,3-dihydro-2,3-dihydroxybenzoate + NAD+ = 2,3-dihydroxybenzoate + NADH + H+ enzyme functions as an alcohol dehydrogenase which oxidizes the C3-hydroxylgroup of the substrate, the transient intermediate 2-hydroxy-3-oxo-4,6-cyclohexadiene-1-carboxylate undergoes rapid aromatization to give 2,3-dihydroxybenzoate Escherichia coli
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.28 66
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.28 (2S,3S)-2,3-dihydro-2,3-dihydroxybenzoate + NAD+
-
 Escherichia coli 2,3-dihydroxybenzoate + NADH + H+
-
 r
1.3.1.28 2-cyclohexen-1-ol + NAD+
-
 Escherichia coli 1-oxo-2-cyclohexene + NADH
-
 r
1.3.1.28 3-hydroxy-4-cyclohexene-1-carboxylate reversible only with 3-keto compounds, not with 2-keto compounds Escherichia coli 3-oxo-4-cyclohexene-1-carboxylate + NADH
-
 r
1.3.1.28 cis-1,3-cyclohexadiene-5,6-diol + NAD+
-
 Escherichia coli ?
-
 r
1.3.1.28 cis-3,5-cyclohexadiene-1,2-diol + NAD+
-
 Escherichia coli ?
-
 r
1.3.1.28 cis-3-hydroxy-4-cyclohexene-1-carboxylate + NAD+
-
 Escherichia coli 3-oxo-4-cyclohexene-1-carboxylate + NADH + H+
-
 r
1.3.1.28 cis-3-hydroxy-cyclohexane-1-carboxylate + NAD+
-
 Escherichia coli 3-oxo-cyclohexane-1-carboxylate + NADH
-
 r
1.3.1.28 methyl 2,3-dihydro-2,3-dihydroxybenzoate + NAD+
-
 Escherichia coli ?
-
 r
1.3.1.28 methyl-2,3-dihydro-3-hydroxybenzoate + NAD+
-
 Escherichia coli 3-hydroxybenzoate + NADH
-
 r
1.3.1.28 methyl-3-hydroxy-1,4-cyclohexadien-1-carboxylate + NAD+
-
 Escherichia coli 3-hydroxybenzoate + NADH
-
 r
1.3.1.28 trans-1,3-cyclohexadiene-5,6-diol + NAD+
-
 Escherichia coli ?
-
 r
1.3.1.28 trans-2,3-dihydro-2,3-dihydroxybenzoate + NAD+
-
 Escherichia coli 2,3-dihydroxybenzoate + NADH
-
 ir
1.3.1.28 trans-3-hydroxy-2-cyclohexene-1-carboxylate + NAD+
-
 Escherichia coli ?
-
 r
1.3.1.28 trans-3-hydroxy-cyclohexane-1-carboxylate + NAD+
-
 Escherichia coli 3-oxo-cyclohexane-1-carboxylate + NADH
-
 r
1.3.1.28 trans-3-hydroxycyclohexane-1-carboxylic acid + NAD+
-
 Escherichia coli 3-oxo-cyclohexane-1-carboxylate + NADH + H+
-
 r

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.1.28 0.733
-
 trans-3-hydroxycyclohexane-1-carboxylic acid
-
 Escherichia coli
1.3.1.28 1
-
 2-Cyclohexen-1-ol
-
 Escherichia coli
1.3.1.28 1
-
 trans-3,5-cyclohexadiene-1,2-diol
-
 Escherichia coli
1.3.1.28 3
-
 methyl-3-hydroxy-1,4-cyclohexadiene-1-carboxylate
-
 Escherichia coli
1.3.1.28 5
-
 trans-3-hydroxy-2-cyclohexene-1-carboxylate
-
 Escherichia coli
1.3.1.28 5
-
 cis-3-hydroxycyclohexane-1-carboxylic acid
-
 Escherichia coli
1.3.1.28 16.7
-
 cis-3,5-cyclohexadiene-1,2-diol
-
 Escherichia coli
1.3.1.28 17.5
-
 methyl 2,3-dihydro-2,3-dihydroxybenzoate
-
 Escherichia coli
1.3.1.28 23
-
 cis-3-hydroxy-4-cyclohexene-1-carboxylate
-
 Escherichia coli
1.3.1.28 92.5
-
 trans-2,3-dihydro-2,3-dihydroxy-benzoate
-
 Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.3.1.28 1.4
-
 cis-2-hydroxy-3-cyclohexen-1-carboxylate
-
 Escherichia coli
1.3.1.28 2.3
-
 trans-2-hydroxycyclohexane-1-carboxylate
-
 Escherichia coli
1.3.1.28 3.3
-
 cis-2-hydroxycyclohexane-1-carboxylate
-
 Escherichia coli