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Literature summary extracted from

  • Fong, K.P.Y.; Goh, C.B.H.; Tan, H.M.
    Characterization and expression of the plasmid-borne bedD gene from Pseudomonas putida ML2, which codes for a NAD+-dependent cis-benzene dihydrodiol dehydrogenase (1996), J. Bacteriol., 178, 5592-5601.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.19 gene bedD encoding cis-benzene dihydrodiol dehydrogenase on the natural plasmid pHMT112, expressed in Escherichia coli Pseudomonas putida
1.3.1.19 sequenze similarity to glycerol dehydrogenase of Escherichia coli Pseudomonas putida
1.3.1.25
-
 Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.19 39000
-
 4 * 39000, SDS-PAGE Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.19 cis-1,2-dihydrobenzene-1,2-diol + NAD+ Pseudomonas putida
-
 catechol + NADH
-
 ?
1.3.1.19 cis-1,2-dihydrobenzene-1,2-diol + NAD+ Pseudomonas putida ML2
-
 catechol + NADH
-
 ?
1.3.1.25 (-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+ Pseudomonas putida enzyme is involved in the metabolism of benzene. A bedD mutant in which the gene is disrupted by a kanamycin resistance cassette is unable to utilize benzene for growth catechol + CO2 + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.19 Pseudomonas putida
-
-
-
1.3.1.19 Pseudomonas putida ML2
-
-
-
1.3.1.25 Pseudomonas putida
-
-
-
1.3.1.25 Pseudomonas putida ML2
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.19 dye-affinity chromatography and NAD+ agarose chromatography Pseudomonas putida
1.3.1.25
-
 Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.25 89.1
-
-
 Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.19 1,2-propanediol + NAD+
-
 Pseudomonas putida propen-1,2-diol + NADH
-
 ?
1.3.1.19 1,2-propanediol + NAD+
-
 Pseudomonas putida ML2 propen-1,2-diol + NADH
-
 ?
1.3.1.19 cis-1,2-cyclohexanediol + NAD+
-
 Pseudomonas putida cyclohex-1-ene-1,2-diol + NADH
-
 ?
1.3.1.19 cis-1,2-cyclohexanediol + NAD+
-
 Pseudomonas putida ML2 cyclohex-1-ene-1,2-diol + NADH
-
 ?
1.3.1.19 cis-1,2-dihydrobenzene-1,2-diol + NAD+
-
 Pseudomonas putida catechol + NADH
-
 ?
1.3.1.19 cis-1,2-dihydrobenzene-1,2-diol + NAD+
-
 Pseudomonas putida ML2 catechol + NADH
-
 ?
1.3.1.25 (-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+ enzyme is involved in the metabolism of benzene. A bedD mutant in which the gene is disrupted by a kanamycin resistance cassette is unable to utilize benzene for growth Pseudomonas putida catechol + CO2 + NADH
-
 ?
1.3.1.25 1,2-propanediol + NAD+ at 189% of the activity with cis-benzene dihydrodiol Pseudomonas putida ?
-
 ?
1.3.1.25 1,2-propanediol + NAD+ at 189% of the activity with cis-benzene dihydrodiol Pseudomonas putida ML2 ?
-
 ?
1.3.1.25 cis-1,2-cyclohexanediol + NAD+ at 95% of the activity with cis-benzene dihydrodiol Pseudomonas putida ?
-
 ?
1.3.1.25 cis-benzene dihydrodiol + NAD+
-
 Pseudomonas putida dihydroxybenzene + NADH
-
 ?
1.3.1.25 glycerol + NAD+ at 18% of the activity with cis-benzene dihydrodiol Pseudomonas putida ?
-
 ?
1.3.1.25 glycerol + NAD+ at 18% of the activity with cis-benzene dihydrodiol Pseudomonas putida ML2 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.19 tetramer 4 * 39000, SDS-PAGE Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
1.3.1.19 cis-benzene dihydrodiol dehydrogenase
-
 Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.19 NAD+
-
 Pseudomonas putida