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Literature summary extracted from

  • Axcell, B.C.; Geary, P.J.
    The metabolism of benzene by bacteria. Purification and some properties of the enzyme cis-1,2-dihydroxycyclohexa-3,5-diene (nicotinamide adenine dinucleotide) oxidoreductase (cis-benzene glycol dehydrogenase) (1973), Biochem. J., 136, 927-934.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.3.1.19 Fe2+
-
 Pseudomonas sp.

General Stability

EC Number General Stability Organism
1.3.1.19 dilution, 0-4°C, sharp fall in activity in the first 5-10 h Pseudomonas sp.
1.3.1.19 loss of activity by ammonium sulfate precipitation and subsequent dialysis, restored by Fe2+ Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.19 1,10-phenanthroline
-
 Pseudomonas sp.
1.3.1.19 p-chloromercuribenzoic acid
-
 Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.19 0.0435
-
 NAD+
-
 Pseudomonas sp.
1.3.1.19 0.286
-
 cis-1,2-dihydrobenzene-1,2-diol cosubstrate NAD+ Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.3.1.19 Fe2+ optimum Fe2+ concentration 0.1 mM Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.19 110000
-
 4 * 110000, SDS-PAGE Pseudomonas sp.
1.3.1.19 440000
-
 ultracentrifugation Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.19 cis-1,2-dihydrobenzene-1,2-diol + NAD+ Pseudomonas sp.
-
 catechol + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.19 Pseudomonas sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.19
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.19 19.1
-
 cis-1,2-dihydrobenzene-1,2-diol, NAD+ Pseudomonas sp.

Storage Stability

EC Number Storage Stability Organism
1.3.1.19 0-4°C or frozen at -22°C, concentrated solution, several weeks Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.19 cis-1,2-dihydrobenzene-1,2-diol + NAD+
-
 Pseudomonas sp. catechol + NADH
-
 ?
1.3.1.19 cis-1,2-dihydrobenzene-1,2-diol + NAD+ specific for the cis-form of benzene glycol and for NAD+ as a hydrogen acceptor Pseudomonas sp. catechol + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.19 tetramer 4 * 110000, SDS-PAGE Pseudomonas sp.

Synonyms

EC Number Synonyms Comment Organism
1.3.1.19 cis-1,2-dihydrocyclohexa-3,5-diene oxidoreductase
-
 Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.19 30
-
 enzyme assay Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.19 7.4
-
 with NAD+ as cofactor and Fe2+ ions, 25 mM potassium phosphate buffer Pseudomonas sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.3.1.19 7.9
-
 in 25 mM-potassium phosphate buffer Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.19 NAD+
-
 Pseudomonas sp.