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Literature summary extracted from

  • Taylor, W.H.; Taylor, M.L.; Eames, D.F.
    Two functionally different dihydroorotic dehydrogenases in bacteria (1966), J. Bacteriol., 91, 2251-2256.
    View publication on PubMedView publication on EuropePMC

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.1.15 soluble
-
 Pseudomonas sp.
-
-
1.3.98.1 particle-bound
-
 Pseudomonas sp.
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.15 orotate + NADPH Pseudomonas sp. degradative enzyme (S)-dihydroorotate + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.15 Pseudomonas sp.
-
 pseudomonad isolated from lake bank mud, capable of growing on salt medium with glucose, aspartate, glycerol or orotate as carbon source
-
1.3.98.1 Pseudomonas sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.98.1 centrifugation after cell disruption, separation from the NADP-linked dihydroorotic dehydrogenase Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.15 0.19
-
 enzyme activity in cells grown on orotate Pseudomonas sp.
1.3.98.1 0.032
-
-
 Pseudomonas sp.

Storage Stability

EC Number Storage Stability Organism
1.3.1.15 -20°C, 4 months, complete loss of activity Pseudomonas sp.
1.3.98.1 -20°C, 4 months Pseudomonas sp.
1.3.98.1 0°C, 1 week Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.15 orotate + NADPH enzyme is 20fold less active with NADH as cofactor Pseudomonas sp. (S)-dihydroorotate + NADP+
-
 ?
1.3.1.15 orotate + NADPH degradative enzyme Pseudomonas sp. (S)-dihydroorotate + NADP+
-
 ?
1.3.98.1 (S)-dihydroorotate + O2 ferricyanide as alternate electron acceptor, enzyme produced regardless of the carbon source Pseudomonas sp. orotate + H2O2
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.15 NADPH
-
 Pseudomonas sp.