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Literature summary extracted from

  • Friedmann, H.C.; Vennesland, B.
    Crystalline dihydroorotic dehydrogenase (1960), J. Biol. Chem., 235, 1526-1530.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.3.1.14 cysteine essential for assay, interaction of enzyme with orotate or dihydroorotate is dependent on cysteine, whereas NADH oxidase activity is not Faecalicatena orotica
1.3.1.14 cysteine required for activation of purified enzyme, but not if crude extract is used for assay Faecalicatena orotica

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.3.1.14
-
 Faecalicatena orotica

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.14 5-Methylorotate 2 mM, 50% inhibition of reduction of orotate Faecalicatena orotica
1.3.1.14 additional information not inhibited by 0.01 M arsenite Faecalicatena orotica
1.3.1.14 NaCl 0.2 M, 55% inhibition Faecalicatena orotica
1.3.1.14 p-chloromercuribenzoate
-
 Faecalicatena orotica
1.3.1.14 phosphate high concentrations Faecalicatena orotica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.14 62000
-
-
 Faecalicatena orotica

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.14 Faecalicatena orotica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.14
-
 Faecalicatena orotica

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.14 additional information
-
-
 Faecalicatena orotica

Storage Stability

EC Number Storage Stability Organism
1.3.1.14 4°C, crystals, over a month, little or no loss of activity Faecalicatena orotica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.14 (S)-dihydroorotate + NAD+ substrate is oxidized by NAD+ and oxygen Faecalicatena orotica orotate + NADH + H+ lipoic acid is no substitute for orotate ?
1.3.1.14 5-fluorodihydroorotate + NAD+ more active substrate for reverse reaction than orotate Faecalicatena orotica 5-fluoroorotate + NADH + H+
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.14 20
-
 assay at Faecalicatena orotica
1.3.1.14 25 30
-
 Faecalicatena orotica

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.1.14 additional information
-
 additional information values not per enzyme molecule, but per mol of flavin Faecalicatena orotica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.14 6.5
-
 maximal activity Faecalicatena orotica

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.14 FAD flavoprotein, ratio FAD to FMN 1:1 Faecalicatena orotica
1.3.1.14 FAD one molecule FAD and one molecule FMN per enzyme molecule Faecalicatena orotica
1.3.1.14 flavin flavoprotein with two different flavins, probably FAD and FMN Faecalicatena orotica
1.3.1.14 flavin 1 mol of flavin per 31 g of protein Faecalicatena orotica
1.3.1.14 FMN flavoprotein, ratio FAD to FMN 1:1 Faecalicatena orotica
1.3.1.14 FMN one molecule FAD and one molecule FMN per enzyme molecule Faecalicatena orotica
1.3.1.14 additional information one atom of iron bound per flavin Faecalicatena orotica