Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Lieberman, I.; Kornberg, A.
    Enzymic synthesis and breakdown of a pyrimidine, orotic acid. I. Dihydro-orotic dehydrogenase (1953), Biochim. Biophys. Acta, 12, 223-234.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.3.1.14 cysteine reaction rate increases 2fold in the presence of 2-7 mM cysteine Faecalicatena orotica

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.14 cysteine inhibitory above 7 mM Faecalicatena orotica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.14 0.11
-
 Orotate
-
 Faecalicatena orotica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.14 dihydroorotate + NAD+ Faecalicatena orotica
-
 orotate + NADH + H+ breakdown of orotate r

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.14 Faecalicatena orotica
-
 obligate anaerobic soil bacterium
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.14 partial purification Faecalicatena orotica

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.14 additional information
-
-
 Faecalicatena orotica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.14 (S)-dihydroorotate + NAD+ equilibrium favours direction of orotate reduction Faecalicatena orotica orotate + NADH + H+ orotate is identical with 4-carboxyuracil r
1.3.1.14 dihydroorotate + NAD+
-
 Faecalicatena orotica orotate + NADH + H+ breakdown of orotate r
1.3.1.14 additional information not: uracil, cytosine, 5-methylcytosine, thymine as substrates Faecalicatena orotica ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.14 6.5
-
 pH of assay medium Faecalicatena orotica

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.3.1.14 5.5 7.8 pH 5.5: 13% of the activity at pH 6.5 Faecalicatena orotica
1.3.1.14 5.5 7.8 pH 7.8: 65% of the activity at pH 6.5 Faecalicatena orotica

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.3.1.14 6.5
-
 more stable than at pH 7.0 Faecalicatena orotica
1.3.1.14 7
-
 less stable than at pH 6.5 Faecalicatena orotica