EC Number | Cloned (Comment) | Organism |
---|---|---|
1.3.1.12 | - |
Escherichia coli |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.3.1.12 | R294Q | chorismate mutase-prephenate dehydrogenase bifunctional enzyme, R294Q substitution reduces the affinity of the enzyme for prephenate, Arg294 interacts electrostatically with the ring carboxylate at C1 of prephenate | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.1.12 | 0.045 | 0.09 | prephenate | - |
Escherichia coli | |
1.3.1.12 | 0.103 | - |
NAD+ | wild-type enzyme | Escherichia coli | |
1.3.1.12 | 0.38 | - |
NAD+ | mutant form R294Q | Escherichia coli | |
1.3.1.12 | 5 | - |
prephenate | mutant form R294Q | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.1.12 | prephenate + NAD+ | Escherichia coli | biosynthesis of L-tyrosine | 4-hydroxyphenylpyruvate + NADH + CO2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.1.12 | Escherichia coli | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.1.12 | prephenate + NAD+ | - |
Escherichia coli | 4-hydroxyphenylpyruvate + NADH + CO2 | - |
? | |
1.3.1.12 | prephenate + NAD+ | biosynthesis of L-tyrosine | Escherichia coli | 4-hydroxyphenylpyruvate + NADH + CO2 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.3.1.12 | chorismate mutase-prephenate dehydrogenase bifunctional enzyme | complex with EC 5.4.99.5 | Escherichia coli |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.1.12 | 0.417 | - |
NAD+ | mutant form R294Q | Escherichia coli | |
1.3.1.12 | 0.417 | - |
prephenate | mutant form R294Q | Escherichia coli | |
1.3.1.12 | 0.45 | - |
NAD+ | wild-type enzyme | Escherichia coli | |
1.3.1.12 | 0.45 | - |
prephenate | wild-type enzyme | Escherichia coli |