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Literature summary extracted from

  • Bhosale, S.B.; Rood, J.I.; Sneddon, M.K.; Morrison, J.F.
    Production of chorismate mutase-prephenate dehydrogenase by a strain of Escherichia coli carrying a multicopy, tyrA plasmid. Isolation and properties of the enzyme (1982), Biochim. Biophys. Acta, 717, 6-11.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.12 overexpression Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.12 78000 100000 chorismate mutase-prephenate dehydrogenase bifunctional enzyme, gel electrophoresis Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.12 prephenate + NAD+ Escherichia coli biosynthesis of L-tyrosine 4-hydroxyphenylpyruvate + NADH + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.12 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.12 chorismate mutase-prephenate dehydrogenase bifunctional enzyme Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.12 52 101
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
1.3.1.12 -20°C, 0.1 M N-ethylmorpholine, pH 7.0, 21 mM citrate, 10% v/v glycerol, 1 mM EDTA, 1 mM DTT Escherichia coli
1.3.1.12 4°C, 0.1 M N-ethylmorpholine, pH 7.0, 21 mM citrate, 10% v/v glycerol, 1 mM EDTA, 1 mM DTT, up to 4 weeks Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.12 prephenate + NAD+
-
 Escherichia coli 4-hydroxyphenylpyruvate + NADH + CO2
-
 ?
1.3.1.12 prephenate + NAD+ biosynthesis of L-tyrosine Escherichia coli 4-hydroxyphenylpyruvate + NADH + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.1.12 chorismate mutase-prephenate dehydrogenase bifunctional enzyme complex with EC 5.4.99.5 Escherichia coli
1.3.1.12 tyrA
-
 Escherichia coli