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Literature summary extracted from
- The metabolism of coumarin by a microorganism. II. The reduction of o-coumaric acid to melilotic acid (1964), Biochemistry, 3, 1944-1947.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.3.1.11 | iodoacetate | 3.3 mM, 29% inhibition | Arthrobacter sp. |  |
| 1.3.1.11 | N-ethylmaleimide | 3.0 mM, 63% inhibition | Arthrobacter sp. | |
| 1.3.1.11 | p-chloromercuribenzoate | 3.0 mM, 100% inhibition, 0.33 mM, 37% inhibition | Arthrobacter sp. | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.3.1.11 | 0.0173 | - |
o-coumarate | - |
Arthrobacter sp. | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.3.1.11 | 2-coumarate + NADH | Arthrobacter sp. | - |
melilotate + NAD+ | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.3.1.11 | Arthrobacter sp. | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.3.1.11 | partially | Arthrobacter sp. |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.3.1.11 | 0.56 | - |
- |
Arthrobacter sp. |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.3.1.11 | 1 week, -20°C | Arthrobacter sp. |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.3.1.11 | 2,4-dihydroxycinnamic acid + NADH | 1.6% of activity with 2-coumarate | Arthrobacter sp. | ? | - |
? | |
| 1.3.1.11 | 2-coumarate + NADH | - |
Arthrobacter sp. | melilotate + NAD+ | - |
? | |
| 1.3.1.11 | 2-coumarate + NADH | - |
Arthrobacter sp. | melilotate + NAD+ | - |
ir | |
| 1.3.1.11 | 4-coumarate + NADH | 1.4% of activity with 2-coumarate | Arthrobacter sp. | ? | - |
? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.3.1.11 | 6.8 | 8.5 | o-coumarate + NADH | Arthrobacter sp. |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.3.1.11 | NAD+ | - |
Arthrobacter sp. | |
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Release 2023.1 (January 2023)
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