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Literature summary extracted from

  • Lorite, M.J.; Tachil, J.; Sanjuan, J.; Meyer, O.; Bedmar, E.J.
    Carbon monoxide dehydrogenase activity in Bradyrhizobium japonicum (2000), Appl. Environ. Microbiol., 66, 1871-1876.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.5.3 iodoacetamide yields di(carboxamidomethyl)molybdopterin cytosine dinucleotide from reaction with the molybdenum cofactor Bradyrhizobium japonicum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.5.3 cytosol
-
 Bradyrhizobium japonicum 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.5.3 Fe2+ 7.6 mol/mol of enzyme Bradyrhizobium japonicum
1.2.5.3 Mo2+ 7.6 mol/mol of enzyme Bradyrhizobium japonicum
1.2.5.3 additional information the enzyme contains 2.29 mol of Mo, 7.96 mol of Fe, 7.60 mol of S, and 1.99 mol of flavins at a 1.15:4:3.82:1 molar ratio, but contains no tungsten Bradyrhizobium japonicum
1.2.7.4 Molybdenum 2.29 mol/mol enzyme Bradyrhizobium japonicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.5.3 17200
-
 (alphabetagamma)2, 2 * 75000, large subunit, + 1 * 28400, medium subunit, + 1 * 17200, small subunit, SDS-PAGE Bradyrhizobium japonicum
1.2.5.3 28400
-
 (alphabetagamma)2, 2 * 75000, large subunit, + 1 * 28400, medium subunit, + 1 * 17200, small subunit, SDS-PAGE Bradyrhizobium japonicum
1.2.5.3 75000
-
 (alphabetagamma)2, 2 * 75000, large subunit, + 1 * 28400, medium subunit, + 1 * 17200, small subunit, SDS-PAGE Bradyrhizobium japonicum
1.2.5.3 230000
-
 native PAGE Bradyrhizobium japonicum
1.2.7.4 17200
-
 L2,M2,S2, 2 * 75000 + 2 * 28400 + 2 * 17200, SDS-PAGE Bradyrhizobium japonicum
1.2.7.4 28400
-
 L2,M2,S2, 2 * 75000 + 2 * 28400 + 2 * 17200, SDS-PAGE Bradyrhizobium japonicum
1.2.7.4 75000
-
 L2,M2,S2, 2 * 75000 + 2 * 28400 + 2 * 17200, SDS-PAGE Bradyrhizobium japonicum
1.2.7.4 230000
-
 native PAGE Bradyrhizobium japonicum
1.2.7.4 235000
-
 gel filtration Bradyrhizobium japonicum

Organism

EC Number Organism UniProt Comment Textmining
1.2.5.3 Bradyrhizobium japonicum
-
-
-
1.2.5.3 Bradyrhizobium japonicum 110spc4
-
-
-
1.2.7.4 Bradyrhizobium japonicum
-
 gram-negativ soil bacterium
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.5.3 native enzyme 21fold from cell-free extract by ultracentrifugation, anion exchange chroomatography, ultrafiltration, hydrophobic interaction chromatography, ultrafiltration, and gel filtration Bradyrhizobium japonicum
1.2.7.4 anion-exchange, hydrophobic interaction, gel filtration Bradyrhizobium japonicum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.5.3 culture condition:CO-grown cell chemolithoautotrophic, CO-dependent growth of strain 110spc4, carbon monoxide as a sole energy and carbon source under aerobic conditions Bradyrhizobium japonicum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.5.3 0.058
-
 purified enzyme, pH 7.5, 30°C Bradyrhizobium japonicum
1.2.7.4 0.0577
-
-
 Bradyrhizobium japonicum

Subunits

EC Number Subunits Comment Organism
1.2.5.3 heterohexamer (alphabetagamma)2, 2 * 75000, large subunit, + 1 * 28400, medium subunit, + 1 * 17200, small subunit, SDS-PAGE Bradyrhizobium japonicum
1.2.7.4 hexamer L2,M2,S2, 2 * 75000 + 2 * 28400 + 2 * 17200, SDS-PAGE Bradyrhizobium japonicum

Synonyms

EC Number Synonyms Comment Organism
1.2.5.3 CODH
-
 Bradyrhizobium japonicum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.5.3 30
-
 assay at Bradyrhizobium japonicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.5.3 7.5
-
 assay at Bradyrhizobium japonicum

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.5.3 flavin
-
 Bradyrhizobium japonicum
1.2.5.3 molybdenum cofactor molybdopterin cytosine dinucleotide as the organic portion of the Bradyrhizobium japonicum CODH molybdenum cofactor Bradyrhizobium japonicum
1.2.5.3 additional information the enzyme contains 2.29 mol of Mo, 7.96 mol of Fe, 7.60 mol of S, and 1.99 mol of flavins at a 1.15:4:3.82:1 molar ratio, but contains no tungsten Bradyrhizobium japonicum
1.2.5.3 phenazine methosulfate artificial electron carrier Bradyrhizobium japonicum
1.2.7.4 flavin adenine dinucleotide 1.99 mol flavin/mol enzyme Bradyrhizobium japonicum
1.2.7.4 molybdopterin cytosine dinucleotide
-
 Bradyrhizobium japonicum