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Literature summary extracted from

  • Grahame, D.A.
    Substrate and cofactor reactivity of a carbon monoxide dehydrogenase-corrinoid enzyme complex: stepwise reduction of iron-sulfur and corrinoid centers, the corrinoid Co2+/1+ redox midpoint potential, and overall synthesis of acetyl-CoA (1993), Biochemistry, 32, 10786-10793.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.169 Fe corrinoid/iron-sulfur protein required Methanosarcina barkeri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.7.4 additional information
-
 the enzyme is part of a high molecular mass multienzyme complex that contains a corrinoid protein and carbon monoxide dehydrogenase and requires tetrahydrosarcinapterin as methyl group acceptor Methanosarcina barkeri

Organism

EC Number Organism UniProt Comment Textmining
1.2.7.4 Methanosarcina barkeri
-
-
-
2.3.1.169 Methanosarcina barkeri
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.169 acetyl-CoA + a [Co(I) corrinoid Fe-S protein] = CO + CoA + a [methyl-Co(III) corrinoid Fe-S protein] pathway Methanosarcina barkeri
a
2.3.1.169 acetyl-CoA + a [Co(I) corrinoid Fe-S protein] = CO + CoA + a [methyl-Co(III) corrinoid Fe-S protein] the corronoid protein functions as methyl group carrier during acetyl-CoA synthesis and decomposition Methanosarcina barkeri
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.7.4 40
-
-
 Methanosarcina barkeri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.7.4 tetrahydrosarcinapterin + acetyl-SCoA + H2O carbon monoxide dehydrogenase-corrinoid enzyme complex, enzyme complex functions in synthesis of acetyl-CoA Methanosarcina barkeri methyltetrahydrosarcinapterin + CO2 + CoASH
-
 r
2.3.1.169 CH3-CO-S-CoA + tetrahydrosarcinapterin + H2O the carbon monoxide dehydrogenase-corrinoid enzyme complex catalyzes the cleavage of acetyl-CoA, tetrahydrosarcinapterin functions as the methyl group acceptor, the major products of reaction are methyltetrahydrosarcinapterin and CO2, free CoA is identified as an additional product Methanosarcina barkeri CH3-tetrahydrosarcinapterin + CO2 + H+ + electron
-
 ?
2.3.1.169 CH3-tetrahydrosarcinapterin + CO + HS-CoA
-
 Methanosarcina barkeri CH3-CO-S-CoA + tetrahydrosarcinapterin
-
 ?
2.3.1.169 CO + H2O
-
 Methanosarcina barkeri CO2 + H+ + electron
-
 r
2.3.1.169 CO + methyl-X + HS-CoA
-
 Methanosarcina barkeri CH3-CO-S-CoA + HX
-
 ?