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Literature summary extracted from

  • Bonam, D.; Ludden, P.W.
    Purification and characterization of carbon monoxide dehydrogenase, a nickel, zinc, iron-sulfur protein, from Rhodospirillum rubrum (1987), J. Biol. Chem., 262, 2980-2987.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.7.4 Iron enzyme form I contains 7 iron and 6 sulfur per monomer Rhodospirillum rubrum
1.2.7.4 Iron enzyme form II contains 9 iron and 8 sulfur per monomer Rhodospirillum rubrum
1.2.7.4 Nickel enzyme form I contains 0.6 mol of nickel per mol of monomer Rhodospirillum rubrum
1.2.7.4 Nickel enzyme form II contains 1.4 mol of nickel per mol of monomer Rhodospirillum rubrum
1.2.7.4 Zinc enzyme form I contains 0.4 mol of zinc per mol of monomer Rhodospirillum rubrum
1.2.7.4 Zinc enzyme form II contains 0.8 mol of zinc per mol of monomer Rhodospirillum rubrum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.7.4 61600
-
gel filtration Rhodospirillum rubrum
1.2.7.4 61800
-
1 * 61800, SDS-PAGE Rhodospirillum rubrum

Organism

EC Number Organism UniProt Comment Textmining
1.2.7.4 Rhodospirillum rubrum
-
purple nonsulfur bacterium, the enzyme exists in the forms, I and II, which differ in metal content
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.7.4
-
Rhodospirillum rubrum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.7.4 culture condition:ammonium malate-grown cell induced with 100% CO 24 h before harvesting Rhodospirillum rubrum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.7.4 694
-
-
Rhodospirillum rubrum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.7.4 CO + H2O + methyl viologen
-
Rhodospirillum rubrum CO2 + reduced methyl viologen
-
?

Subunits

EC Number Subunits Comment Organism
1.2.7.4 monomer 1 * 61800, SDS-PAGE Rhodospirillum rubrum