EC Number | General Stability | Organism |
---|---|---|
1.2.1.8 | at the optimum pH of 8.0 the enzyme is inactivated by dilution, it is stable at pH 6.5 even at very low concentrations | Pseudomonas aeruginosa |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.8 | Betaine aldehyde | - |
Pseudomonas aeruginosa |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.2.1.8 | 61000 | - |
2 * 61000, SDS-PAGE | Pseudomonas aeruginosa |
1.2.1.8 | 139000 | - |
gel filtration | Pseudomonas aeruginosa |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.8 | betaine aldehyde + NAD+ + H2O | Pseudomonas aeruginosa | enzyme catalyzes the last, irreversible step in the synthesis of the osmoprotectant glycine betaine from choline, also obligatory step in the assimilation of carbon and nitrogen when bacteria are growing in choline or choline precursors | betaine + NADH | - |
ir |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.1.8 | Pseudomonas aeruginosa | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.2.1.8 | rapid purification | Pseudomonas aeruginosa |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.2.1.8 | 146.3 | - |
- |
Pseudomonas aeruginosa |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.8 | 4-aminobutyraldehyde + NAD+ + H2O | no activity | Pseudomonas aeruginosa | 4-aminobutyrate + NADH | - |
? | |
1.2.1.8 | betaine aldehyde + NAD+ + H2O | preferentially uses NADP+ over NAD+ | Pseudomonas aeruginosa | betaine + NADH | - |
ir | |
1.2.1.8 | betaine aldehyde + NAD+ + H2O | enzyme catalyzes the last, irreversible step in the synthesis of the osmoprotectant glycine betaine from choline, also obligatory step in the assimilation of carbon and nitrogen when bacteria are growing in choline or choline precursors | Pseudomonas aeruginosa | betaine + NADH | - |
ir | |
1.2.1.8 | betaine aldehyde + NADP+ + H2O | preferentially uses NADP+ over NAD+ | Pseudomonas aeruginosa | betaine + NADPH | - |
? | |
1.2.1.8 | additional information | no activity with 3-dimethylsulfoniopropionaldehyde | Pseudomonas aeruginosa | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.2.1.8 | dimer | 2 * 61000, SDS-PAGE | Pseudomonas aeruginosa |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.2.1.8 | 40 | - |
loss of activity, activity is regained, when the heated enzyme is cooled to 30°C or lower | Pseudomonas aeruginosa |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.2.1.8 | 8 | - |
Tris-HCl buffer | Pseudomonas aeruginosa |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.2.1.8 | 7 | 9.5 | pH 7.0: about 50% of maximal activity, pH 9.5: about 75% of maximal activity | Pseudomonas aeruginosa |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.8 | NAD+ | - |
Pseudomonas aeruginosa | |
1.2.1.8 | NADP+ | preferentially uses NADP+ over NAD+ | Pseudomonas aeruginosa |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.1.8 | 4.6 | - |
Betaine aldehyde | - |
Pseudomonas aeruginosa |