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Literature summary extracted from

  • Incharoensakdi, A.; Matsuda, N.; Hibino, T.; Meng, Y.L.; Ishikawa, H.; Hara, A.; Funaguma, T.; Takabe, T.; Takabe, T.
    Overproduction of spinach betaine aldehyde dehydrogenase in Escherichia coli. Structural and functional properties of wild-type, mutants and E. coli enzymes (2000), Eur. J. Biochem., 267, 7015-7023.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.8 of genes encoding five forms of spinach enzyme: full length, mature, mutant E103Q, mutant E103K and chimera Spinacia oleracea
1.2.1.8 overexpression in Escherichia coli Spinacia oleracea

Protein Variants

EC Number Protein Variants Comment Organism
1.2.1.8 E103K mutant enzyme has no activity with betaine aldehyde and 4-aminobutyraldehyde Spinacia oleracea
1.2.1.8 E103Q Km-values for 4-aminobutyraldehyde increases compared to wild-type Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.8 0.003
-
 4-Aminobutyraldehyde wild-type enzyme Spinacia oleracea
1.2.1.8 0.0098
-
 4-Aminobutyraldehyde mutant enzyme E103Q Spinacia oleracea
1.2.1.8 0.023
-
 NAD+ mutant enzyme E103Q, reaction with betaine aldehyde Spinacia oleracea
1.2.1.8 0.024
-
 NAD+ mutant enzyme E103Q, reaction with 4-aminobutyraldehyde Spinacia oleracea
1.2.1.8 0.026
-
 NAD+ wild-type enzyme, reaction with 4-aminopropionaldehyde Spinacia oleracea
1.2.1.8 0.037
-
 4-Aminobutyraldehyde
-
 Spinacia oleracea
1.2.1.8 0.065
-
 Betaine aldehyde mutant enzyme E103Q Spinacia oleracea
1.2.1.8 0.068
-
 Betaine aldehyde wild-type enzyme Spinacia oleracea
1.2.1.8 0.07716
-
 Betaine aldehyde
-
 Spinacia oleracea
1.2.1.8 0.07799
-
 NAD+
-
 Spinacia oleracea
1.2.1.8 0.136
-
 NAD+ reaction with 4-aminobutyraldehyde Spinacia oleracea

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.8 Spinacia oleracea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.8
-
 Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.8 4-aminobutyraldehyde + NAD+ + H2O
-
 Spinacia oleracea 4-aminobutyrate + NADH
-
 ?
1.2.1.8 betaine aldehyde + NAD+ + H2O
-
 Spinacia oleracea betaine + NADH
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.8 NAD+
-
 Spinacia oleracea