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Literature summary extracted from

  • Mori, N.; Kawakami, B.; Hyakutome, K.; Tani, Y.; Yamada, H.
    Characterization of betaine aldehyde dehydrogenase from Cylindrocarpon didynum M-1 (1980), Agric. Biol. Chem., 44, 3015-3016.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.2.1.8 complete loss of activity when dialyzed against 10 mM buffer, pH 8.0, addition of 20% glycerol stabilizes Cylindrocarpon didymum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.8 1,10-phenanthroline
-
 Cylindrocarpon didymum
1.2.1.8 2,2'-dipyridyl
-
 Cylindrocarpon didymum
1.2.1.8 Co2+
-
 Cylindrocarpon didymum
1.2.1.8 Cu2+
-
 Cylindrocarpon didymum
1.2.1.8 Hg2+
-
 Cylindrocarpon didymum
1.2.1.8 iodoacetate
-
 Cylindrocarpon didymum
1.2.1.8 PCMB
-
 Cylindrocarpon didymum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.8 0.31
-
 Betaine aldehyde
-
 Cylindrocarpon didymum
1.2.1.8 0.38
-
 NAD+
-
 Cylindrocarpon didymum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.8 58000
-
 4 * 58000, SDS-PAGE Cylindrocarpon didymum
1.2.1.8 220000
-
 gel filtration Cylindrocarpon didymum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.8 betaine aldehyde + NAD+ + H2O Cylindrocarpon didymum synthesis of betaine betaine + NADH
-
 ?
1.2.1.8 betaine aldehyde + NAD+ + H2O Cylindrocarpon didymum M-1 synthesis of betaine betaine + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.8 Cylindrocarpon didymum
-
 M-1
-
1.2.1.8 Cylindrocarpon didymum M-1
-
 M-1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.8
-
 Cylindrocarpon didymum

Reaction

EC Number Reaction Comment Organism Reaction ID
1.2.1.8 betaine aldehyde + NAD+ + H2O = betaine + NADH + 2 H+ ping-pong mechanism Cylindrocarpon didymum
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.8 20.5
-
-
 Cylindrocarpon didymum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.8 betaine aldehyde + NAD+ + H2O
-
 Cylindrocarpon didymum betaine + NADH
-
 ?
1.2.1.8 betaine aldehyde + NAD+ + H2O synthesis of betaine Cylindrocarpon didymum betaine + NADH
-
 ?
1.2.1.8 betaine aldehyde + NAD+ + H2O
-
 Cylindrocarpon didymum M-1 betaine + NADH
-
 ?
1.2.1.8 betaine aldehyde + NAD+ + H2O synthesis of betaine Cylindrocarpon didymum M-1 betaine + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.8 tetramer 4 * 58000, SDS-PAGE Cylindrocarpon didymum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.8 8.5 9
-
 Cylindrocarpon didymum

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.8 NAD+
-
 Cylindrocarpon didymum