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Literature summary extracted from

  • Falkenberg, P.; Strom, A.R.
    Purification and characterization of osmoregulatory betaine aldehyde dehydrogenase of Escherichia coli (1990), Biochim. Biophys. Acta, 1034, 253-259.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.2.1.8 salt-tolerant enzyme retains 50% of its initial activity at 1.2 M K+ Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.8 acetaldehyde 100 mM, 83% inhibition Escherichia coli
1.2.1.8 benzaldehyde 1 mM, 51% inhibition Escherichia coli
1.2.1.8 Betaine aldehyde above 0.5 mM Escherichia coli
1.2.1.8 Butyrylcholine 100 mM, 73% inhibition Escherichia coli
1.2.1.8 choline 100 mM, 78% inhibition Escherichia coli
1.2.1.8 ethanolamine 100 mM, 35% inhibition Escherichia coli
1.2.1.8 glyceraldehyde 100 mM, 83% inhibition Escherichia coli
1.2.1.8 Hg2+ 0.0001-0.0005 mM HgCl2 Escherichia coli
1.2.1.8 iodoacetamide 1 mM Escherichia coli
1.2.1.8 iodoacetate 1 mM Escherichia coli
1.2.1.8 isopentanal 1 mM, 84% inhibition Escherichia coli
1.2.1.8 additional information no substrate inhibition with NAD+ Escherichia coli
1.2.1.8 N,N-dimethylethanolamine 100 mM, 57% inhibition Escherichia coli
1.2.1.8 N,N-dimethylglycine 1 mM, 24% inhibition Escherichia coli
1.2.1.8 N-Methylethanolamine 100 mM, 50% inhibition Escherichia coli
1.2.1.8 N-methylglycine 1 mM, 24% inhibition Escherichia coli
1.2.1.8 NADP+ substrate inhibition above 10 mM Escherichia coli
1.2.1.8 PCMB 0.0001-0.0005 mM Escherichia coli
1.2.1.8 phenylacetaldehyde 1 mM, 54% inhibition Escherichia coli
1.2.1.8 tetramethylammonium hydroxide 100 mM, 57% inhibition Escherichia coli
1.2.1.8 trimethylacetaldehyde 100 mM, 89% inhibition Escherichia coli
1.2.1.8 ZnCl2 0.0001-0.0005 mM Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.8 0.099
-
NAD+
-
Escherichia coli
1.2.1.8 0.16
-
Betaine aldehyde
-
Escherichia coli
1.2.1.8 0.4
-
NADP+
-
Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.8 K+ activation up to 0.15 M, decreases activity at higher concentrations Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.8 55000
-
4 * 55000, SDS-PAGE Escherichia coli
1.2.1.8 232000
-
gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.8 betaine aldehyde + NAD+ + H2O Escherichia coli the salt tolerance is an essential property for the enzyme participating in the cellular synthesis of an osmoprotectant betaine + NADH
-
ir

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.8 Escherichia coli
-
over-producing strain carrying the structural gene for the enzyme on the plasmid pBR322
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.8
-
Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.8 38
-
-
Escherichia coli

Storage Stability

EC Number Storage Stability Organism
1.2.1.8 -80°C, frozen in presence of 10 mM dithiothreitol, stable for several months without appreciable loss of activity Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.8 betaine aldehyde + NAD+ + H2O highly specific for betaine aldehyde Escherichia coli betaine + NADH
-
ir
1.2.1.8 betaine aldehyde + NAD+ + H2O the salt tolerance is an essential property for the enzyme participating in the cellular synthesis of an osmoprotectant Escherichia coli betaine + NADH
-
ir
1.2.1.8 betaine aldehyde + NADP+ + H2O NAD+ is preferred over NADP+ Escherichia coli betaine + NADPH
-
?

Subunits

EC Number Subunits Comment Organism
1.2.1.8 tetramer 4 * 55000, SDS-PAGE Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.8 7.5 9.5
-
Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.8 NAD+ NAD+ is preferred over NADP+ Escherichia coli
1.2.1.8 NADP+ NAD+ is preferred over NADP+ Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.2.1.8 0.1
-
isopentanal
-
Escherichia coli
1.2.1.8 0.53
-
phenylacetaldehyde
-
Escherichia coli
1.2.1.8 0.7
-
benzaladehyde
-
Escherichia coli
1.2.1.8 4.5
-
trimethylacetaldehyde
-
Escherichia coli
1.2.1.8 5.7
-
acetaldehyde
-
Escherichia coli
1.2.1.8 7.4
-
glyceraldehyde
-
Escherichia coli
1.2.1.8 11
-
choline
-
Escherichia coli
1.2.1.8 14
-
Butyrylcholine
-
Escherichia coli
1.2.1.8 26
-
tetramethylammonium hydroxide
-
Escherichia coli
1.2.1.8 27
-
N,N-dimethylethanolamine
-
Escherichia coli
1.2.1.8 40
-
N-Methylethanolamine
-
Escherichia coli
1.2.1.8 78
-
ethanolamine
-
Escherichia coli
1.2.1.8 130
-
N,N-dimethylglycine
-
Escherichia coli
1.2.1.8 130
-
N-methylglycine
-
Escherichia coli