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Literature summary extracted from

  • Krishna, R.V.; Beilstein, P.; Leisinger, T.
    Biosynthesis of proline in Pseudomonas aeruginosa. Properties of gamma-glutamyl phosphate reductase and 1-pyrroline-5-carboxylate reductase (1979), Biochem. J., 181, 223-230.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.2 3-acetylpyridine analogue of NAD+ 95% inhibition at 2 mM Pseudomonas aeruginosa
1.5.1.2 Ag+ inactivation at 0.1 mM Pseudomonas aeruginosa
1.5.1.2 Hg2+ 75% inactivation at 0.1 mM Pseudomonas aeruginosa
1.5.1.2 iodoacetamide complete inactivation at 1 mM Pseudomonas aeruginosa
1.5.1.2 L-2-acetidine-4-carboxylic acid 50% inactivation at 2 mM Pseudomonas aeruginosa
1.5.1.2 p-chloromercuribenzoate complete inactivation at 1 mM Pseudomonas aeruginosa
1.5.1.2 proline 75% inactivation at 50 mM Pseudomonas aeruginosa
1.5.1.2 thio-NAD+ 65% inactivation at 0.2 mM Pseudomonas aeruginosa
1.5.1.2 thio-NADP+ 65% inactivation at 0.2 mM Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.2 0.02
-
 NADPH
-
 Pseudomonas aeruginosa
1.5.1.2 0.05
-
 NADH
-
 Pseudomonas aeruginosa
1.5.1.2 0.09
-
 1-pyrroline-5-carboxylate with NADH Pseudomonas aeruginosa
1.5.1.2 0.12
-
 1-pyrroline-5-carboxylate with NADPH Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.2 94000
-
 sucrose density gradient sedimentation, gel filtration Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.2 pyrroline-5-carboxylate + NAD(P)H + H+ Pseudomonas aeruginosa
-
 L-proline + NAD(P)+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.2 Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.2 partial Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.2 additional information
-
-
 Pseudomonas aeruginosa
1.5.1.2 3317
-
-
 Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.2 1-pyrroline-5-carboxylate + NAD(P)H + H+ specific for L-isomer Pseudomonas aeruginosa L-proline + NAD(P)+
-
 ir
1.5.1.2 1-pyrroline-5-carboxylate + NADH + H+
-
 Pseudomonas aeruginosa L-proline + NAD+
-
 r
1.5.1.2 1-pyrroline-5-carboxylate + NADPH + H+
-
 Pseudomonas aeruginosa L-proline + NADP+
-
 r
1.5.1.2 pyrroline-5-carboxylate + NAD(P)H + H+
-
 Pseudomonas aeruginosa L-proline + NAD(P)+
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.2 6.2 6.5
-
 Pseudomonas aeruginosa

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.2 NADH preferentially Pseudomonas aeruginosa
1.5.1.2 NADPH
-
 Pseudomonas aeruginosa