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Literature summary extracted from

  • Moffa, D.J.; Lotspeich, F.J.; Krause, R.F.
    Preparation and properties of retinal-oxidizing enzyme from rat intestinal mucosa (1970), J. Biol. Chem., 245, 439-447.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.36 glutathione reduced form, reaction rate is maximal, with reduced glutathione Rattus norvegicus

General Stability

EC Number General Stability Organism
1.2.1.36 dialysis against distilled water, several hours, about 29% loss of activity Rattus norvegicus
1.2.1.36 quite unstable during extensive freezing and thawing Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.36 2-Iodoacetamide slight Rattus norvegicus
1.2.1.36 alpha,alpha'-dipyridyl slight Rattus norvegicus
1.2.1.36 Atabrine slight, FAD reverses inhibition Rattus norvegicus
1.2.1.36 FeCl3
-
 Rattus norvegicus
1.2.1.36 NaCN slight Rattus norvegicus
1.2.1.36 NADH inhibits noncompetitively, inhibition not reversed by direct addition of NAD+ Rattus norvegicus
1.2.1.36 p-chloromercuribenzoate
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.36 0.3
-
 retinal
-
 Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.36 Fe2+ reaction rate is maximal with Fe2+ Rattus norvegicus
1.2.1.36 Iron metalloprotein, approximately 2 mol of iron per mol of enzyme Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.36 80000
-
 thin layer chromatography, gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.36 retinal + NAD+ + H2O Rattus norvegicus acts on both the all-trans- and 13-cis-form of retinal, all-trans retinal more actively oxidized than 13-cis retinal retinoate + NADH
-
 ir
1.2.1.36 retinal + NAD+ + H2O Rattus norvegicus Wistar acts on both the all-trans- and 13-cis-form of retinal, all-trans retinal more actively oxidized than 13-cis retinal retinoate + NADH
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.36 Rattus norvegicus
-
 Wistar strain
-
1.2.1.36 Rattus norvegicus Wistar
-
 Wistar strain
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.2.1.36 no glycoprotein
-
 Rattus norvegicus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.36
-
 Rattus norvegicus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.2.1.36 retinal + NAD+ + H2O = retinoate + NADH + H+ acts on both the all-trans- and 13-cis-form of retinal Rattus norvegicus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.36 intestinal mucosa
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.36 0.12
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.36 retinal + NAD+ + H2O acts on both the all-trans- and 13-cis-form of retinal, all-trans retinal more actively oxidized than 13-cis retinal Rattus norvegicus retinoate + NADH
-
 ir
1.2.1.36 retinal + NAD+ + H2O acts on both the all-trans- and 13-cis-form of retinal, all-trans retinal more actively oxidized than 13-cis retinal Rattus norvegicus Wistar retinoate + NADH
-
 ir

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.36 37
-
 assay at 37°C Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.36 7.7
-
 in 0.1 M sodium phosphate buffer, rather broad pH curve with pH-otimum at approximately pH 7.7 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.36 FAD metalloflavoprotein, reaction rate is maximal with FAD Rattus norvegicus
1.2.1.36 NAD+ reaction rate is maximal with NAD+, stimulation by NAD+ reversed by direct addition of NADH Rattus norvegicus