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Literature summary extracted from

  • Shaw, J.P.; Schwager, F.; Harayama, S.
    Substrate-specificity of benzyl alcohol dehydrogenase and benzaldehyde dehydrogenase encoded by TOL plasmid pWW0 (1992), Biochem. J., 283, 789-794.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.32 2-Chlorobenzaldehyde competitive Pseudomonas putida
1.2.1.32 2-Methoxybenzaldehyde competitive Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.32 0.17
-
 3-Fluorobenzaldehyde
-
 Pseudomonas putida
1.2.1.32 0.31
-
 4-nitrobenzaldehyde
-
 Pseudomonas putida
1.2.1.32 0.31
-
 4-Fluorobenzaldehyde
-
 Pseudomonas putida
1.2.1.32 0.48
-
 2-nitrobenzaldehyde
-
 Pseudomonas putida
1.2.1.32 0.53
-
 4-methylbenzaldehyde
-
 Pseudomonas putida
1.2.1.32 0.63
-
 3-methylbenzaldehyde
-
 Pseudomonas putida
1.2.1.32 0.7
-
 4-Chlorobenzaldehyde
-
 Pseudomonas putida
1.2.1.32 0.81
-
 3-Nitrobenzaldehyde
-
 Pseudomonas putida
1.2.1.32 0.94
-
 3-Methoxybenzaldehyde
-
 Pseudomonas putida
1.2.1.32 1.1
-
 4-methoxybenzaldehyde
-
 Pseudomonas putida
1.2.1.32 1.3
-
 3-Chlorobenzaldehyde
-
 Pseudomonas putida
1.2.1.32 2
-
 2-Fluorobenzaldehyde
-
 Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.32 Pseudomonas putida
-
 carrying the TOL plasmid pWW0
-
1.2.1.32 Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182
-
 carrying the TOL plasmid pWW0
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.32 erroneously identified as benzaldehyde dehydrogenase Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.32 2-fluorobenzaldehyde + NAD+ + H2O low activity Pseudomonas putida 2-fluorobenzoate + NADH + H+
-
 ?
1.2.1.32 2-fluorobenzaldehyde + NAD+ + H2O low activity Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 2-fluorobenzoate + NADH + H+
-
 ?
1.2.1.32 2-nitrobenzaldehyde + NAD+ + H2O
-
 Pseudomonas putida 2-nitrobenzoate + NADH + H+
-
 r
1.2.1.32 3-chlorobenzaldehyde + NAD+ + H2O low activity Pseudomonas putida 3-chlorobenzoate + NADH
-
 ?
1.2.1.32 3-fluorobenzaldehyde + NAD+ + H2O low activity Pseudomonas putida 3-fluorobenzoate + NADH + H+
-
 ?
1.2.1.32 3-methoxybenzaldehyde + NAD+ + H2O low activity Pseudomonas putida 3-methoxybenzoate + NADH
-
 ?
1.2.1.32 3-methylbenzaldehyde + NAD+ + H2O low activity Pseudomonas putida 3-methylbenzoate + NADH
-
 ?
1.2.1.32 3-nitrobenzaldehyde + NAD+ + H2O
-
 Pseudomonas putida 3-nitrobenzoate + NADH
-
 ?
1.2.1.32 4-chlorobenzaldehyde + NAD+ + H2O low activity Pseudomonas putida 4-chlorobenzoate + NADH
-
 ?
1.2.1.32 4-chlorobenzaldehyde + NAD+ + H2O low activity Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 4-chlorobenzoate + NADH
-
 ?
1.2.1.32 4-fluorobenzaldehyde + NAD+ + H2O low activity Pseudomonas putida 4-fluorobenzoate + NADH + H+
-
 ?
1.2.1.32 4-fluorobenzaldehyde + NAD+ + H2O low activity Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 4-fluorobenzoate + NADH + H+
-
 ?
1.2.1.32 4-methoxybenzaldehyde + NAD+ + H2O low activity Pseudomonas putida 4-methoxybenzoate + NADH + H+
-
 ?
1.2.1.32 4-methylbenzaldehyde + NAD+ + H2O low activity Pseudomonas putida 4-methylbenzoate + NADH
-
 ?
1.2.1.32 4-nitrobenzaldehyde + NAD+ + H2O low activity Pseudomonas putida 4-nitrobenzoate + NADH + H+
-
 ?
1.2.1.32 additional information no reaction with 2-methylbenzaldehyde Pseudomonas putida ?
-
 ?
1.2.1.32 additional information no reaction with 2-chlorobenzaldehyde Pseudomonas putida ?
-
 ?
1.2.1.32 additional information no reaction with 2-methylbenzaldehyde Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 ?
-
 ?
1.2.1.32 additional information no reaction with 2-chlorobenzaldehyde Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 ?
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.32 NAD+
-
 Pseudomonas putida

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.2.1.32 0.06
-
 2-Methoxybenzaldehyde
-
 Pseudomonas putida
1.2.1.32 0.13
-
 2-Chlorobenzaldehyde
-
 Pseudomonas putida