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Literature summary extracted from

  • Schell, M.A.
    Cloning and expression in Escherichia coli of the naphthalene degradation genes from plasmid NAH7 (1983), J. Bacteriol., 153, 822-829.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.32 NAH plasmid of Pseudomonas putida, carrying the coding gene nahI, cloned into Escherichia coli Pseudomonas putida
1.3.1.29 expression in Escherichia coli and Pseudomonas putida Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.32 Pseudomonas putida
-
-
-
1.3.1.29 Pseudomonas putida
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.29 0.03
-
recombinant enzyme expressed in Escherichia coli, induction with salicylate Pseudomonas putida
1.3.1.29 0.39
-
recombinant enzyme expressed in Pseudomonas putida strain AC10 Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.29 cis-(1R,2S)-dihydroxy-1,2-dihydronaphthalene + NAD+
-
Pseudomonas putida 1,2-dihydroxynaphthalene + NADH
-
?

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.32 NAD+
-
Pseudomonas putida
1.3.1.29 NAD+
-
Pseudomonas putida