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Literature summary extracted from

  • Chalmers, R.M.; Fewson, C.A.
    Purification and characterization of benzaldehyde dehydrogenase I from Acinetobacter calcoaceticus [published erratum appears in Biochem J 1990 Feb 1;265(6):931] (1989), Biochem. J., 263, 913-919.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.28 4-chloromercuribenzoate 0.10 mM, 50% inhibition after less than 1 min, benzaldehyde dehydrogenase I Acinetobacter calcoaceticus
1.2.1.28 benzaldehyde substrate inhibition at concentrations below 0.005 mM, benzaldehyde dehydrogenase I Acinetobacter calcoaceticus
1.2.1.28 iodoacetamide 10 mM, 50% inhibition after less than 1 min, benzaldehyde dehydrogenase I Acinetobacter calcoaceticus
1.2.1.28 iodoacetate 10 mM, 50% inhibition after 11 min, benzaldehyde dehydrogenase I Acinetobacter calcoaceticus
1.2.1.28 N-ethylmaleimide 10 mM, 50% inhibition after less than 1 min, benzaldehyde dehydrogenase I Acinetobacter calcoaceticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.28 0.00057
-
benzaldehyde
-
Acinetobacter calcoaceticus
1.2.1.28 0.079
-
NAD+
-
Acinetobacter calcoaceticus
1.2.1.28 11.2
-
K+
-
Acinetobacter calcoaceticus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.28 K+ or NH4+ or Rb+ required for maximal activity, maximum activity occurs at approx. 70 mM K+ Acinetobacter calcoaceticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.28 56000
-
4 * 56000, dehydrogenase I, SDS-PAGE Acinetobacter calcoaceticus
1.2.1.28 141000 219000 dehydrogenase I, gel filtration with different gels Acinetobacter calcoaceticus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.28 benzaldehyde + NAD+ + H2O Acinetobacter calcoaceticus
-
benzoate + NADH + H+
-
ir

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.28 Acinetobacter calcoaceticus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.28 dehydrogenase I Acinetobacter calcoaceticus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.28 25.4
-
-
Acinetobacter calcoaceticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.28 2-bromobenzaldehyde + NAD+ + H2O
-
Acinetobacter calcoaceticus 2-bromobenzoate + NADH
-
?
1.2.1.28 2-fluorobenzaldehyde + NAD+ + H2O 41% of activity with benzaldehyde Acinetobacter calcoaceticus 2-fluorobenzoate + NADH + H+
-
?
1.2.1.28 2-hydroxybenzaldehyde + NAD+ + H2O 39% of activity with benzaldehyde Acinetobacter calcoaceticus 2-hydroxybenzoate + NADH + H+
-
?
1.2.1.28 2-methoxybenzaldehyde + NAD+ + H2O
-
Acinetobacter calcoaceticus 2-methoxybenzoate + NADH + H+
-
?
1.2.1.28 2-methylbenzaldehyde + NAD+ + H2O 13% of activity with benzaldehyde Acinetobacter calcoaceticus 2-methylbenzoate + NADH
-
?
1.2.1.28 3,4-dihydroxybenzaldehyde + NAD+ + H2O
-
Acinetobacter calcoaceticus 3,4-dihydroxybenzoate + NADH + H+
-
?
1.2.1.28 3-fluorobenzaldehyde + NAD+ + H2O 98% of activity with benzaldehyde Acinetobacter calcoaceticus 3-fluorobenzoate + NADH + H+
-
?
1.2.1.28 3-hydroxybenzaldehyde + NAD+ + H2O 222% of activity with benzaldehyde Acinetobacter calcoaceticus 3-hydroxybenzoate + NADH + H+
-
?
1.2.1.28 3-methoxy-4-hydroxybenzaldehyde + NAD+ + H2O
-
Acinetobacter calcoaceticus 3-methoxy-4-hydroxybenzoate + NADH + H+
-
?
1.2.1.28 3-methoxybenzaldehyde + NAD+ + H2O 235% of activity with benzaldehyde Acinetobacter calcoaceticus 3-methoxybenzoate + NADH
-
?
1.2.1.28 3-methylbenzaldehyde + NAD+ + H2O 119% of activity with benzaldehyde Acinetobacter calcoaceticus 3-methylbenzoate + NADH
-
?
1.2.1.28 4-fluorobenzaldehyde + NAD+ + H2O 210% of activity with benzaldehyde Acinetobacter calcoaceticus 4-fluorobenzoate + NADH + H+
-
?
1.2.1.28 4-hydroxybenzaldehyde + NAD+ + H2O
-
Acinetobacter calcoaceticus 4-hydroxybenzoate + NADH + H+
-
?
1.2.1.28 4-methoxy-3-hydroxybenzaldehyde + NAD+ + H2O
-
Acinetobacter calcoaceticus 4-methoxy-3-hydroxybenzoate + NADH + H+
-
?
1.2.1.28 4-methylbenzaldehyde + NAD+ + H2O 240% of activity with benzaldehyde Acinetobacter calcoaceticus 4-methylbenzoate + NADH
-
?
1.2.1.28 benzaldehyde + NAD+ + H2O
-
Acinetobacter calcoaceticus benzoate + NADH + H+
-
ir
1.2.1.28 benzaldehyde + NAD+ + H2O benzaldehyde dehydrogenase I exhibits esterase activity, approx. 2% of dehydrogenase activity Acinetobacter calcoaceticus benzoate + NADH + H+
-
ir
1.2.1.28 benzaldehyde + NAD+ + H2O also active with NADP+, see also EC 1.2.1.7 Acinetobacter calcoaceticus benzoate + NADH + H+
-
ir
1.2.1.28 hexan-1-al + NAD+ + H2O
-
Acinetobacter calcoaceticus hexanoate + NADH
-
?
1.2.1.28 octan-1-al + NAD+ + H2O
-
Acinetobacter calcoaceticus octanoate + NADH
-
?
1.2.1.28 perillaldehyde + NAD+ + H2O
-
Acinetobacter calcoaceticus perillic acid + NADH
-
?

Subunits

EC Number Subunits Comment Organism
1.2.1.28 tetramer 4 * 56000, dehydrogenase I, SDS-PAGE Acinetobacter calcoaceticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.28 9.5
-
-
Acinetobacter calcoaceticus

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.28 NAD+
-
Acinetobacter calcoaceticus
1.2.1.28 NADP+ 6% activity of NAD+ Acinetobacter calcoaceticus