EC Number | General Stability | Organism |
---|---|---|
1.2.1.28 | dithiothreitol stabilizes | Pseudomonas putida |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.1.28 | 0.0015 | - |
4-methylbenzaldehyde | - |
Pseudomonas putida | |
1.2.1.28 | 0.0019 | - |
3-methylbenzaldehyde | - |
Pseudomonas putida | |
1.2.1.28 | 0.0021 | - |
3-Chlorobenzaldehyde | - |
Pseudomonas putida | |
1.2.1.28 | 0.0025 | - |
benzaldehyde | - |
Pseudomonas putida | |
1.2.1.28 | 0.0026 | - |
3-Methoxybenzaldehyde | - |
Pseudomonas putida | |
1.2.1.28 | 0.0029 | - |
3-Fluorobenzaldehyde | - |
Pseudomonas putida | |
1.2.1.28 | 0.022 | - |
3-Nitrobenzaldehyde | - |
Pseudomonas putida | |
1.2.1.28 | 0.32 | - |
NAD+ | - |
Pseudomonas putida | |
1.2.1.32 | 0.0093 | - |
2-hydroxy-5-methyl-6-oxohexa-2,4-dienoate | synonym 5-methyl-2-hydroxymuconic-6-semialdehyde | Pseudomonas putida | |
1.2.1.32 | 0.017 | - |
2-hydroxymuconate-6-semialdehyde | - |
Pseudomonas putida | |
1.2.1.32 | 0.17 | - |
3-Fluorobenzaldehyde | - |
Pseudomonas putida | |
1.2.1.32 | 0.33 | - |
NAD+ | - |
Pseudomonas putida | |
1.2.1.32 | 0.46 | - |
benzaldehyde | - |
Pseudomonas putida | |
1.2.1.32 | 0.53 | - |
4-methylbenzaldehyde | - |
Pseudomonas putida | |
1.2.1.32 | 0.63 | - |
3-methylbenzaldehyde | - |
Pseudomonas putida | |
1.2.1.32 | 0.81 | - |
3-Nitrobenzaldehyde | - |
Pseudomonas putida | |
1.2.1.32 | 0.94 | - |
3-Methoxybenzaldehyde | - |
Pseudomonas putida | |
1.2.1.32 | 1.3 | - |
3-Chlorobenzaldehyde | - |
Pseudomonas putida | |
1.2.1.85 | 0.0093 | - |
2-hydroxy-5-methyl-6-oxohexa-2,4-dienoate | in 100 mM Tris-HCl, pH 8.5, at 25°C | Pseudomonas putida | |
1.2.1.85 | 0.017 | - |
2-hydroxymuconate-6-semialdehyde | in 100 mM Tris-HCl, pH 8.5, at 25°C | Pseudomonas putida | |
1.2.1.85 | 0.17 | - |
3-Fluorobenzaldehyde | in 100 mM glycine-NaOH (pH 9.2), at 25°C | Pseudomonas putida | |
1.2.1.85 | 0.33 | - |
NAD+ | in the presence of benzaldehyde, 100 mM glycine-NaOH (pH 9.2), at 25°C | Pseudomonas putida | |
1.2.1.85 | 0.46 | - |
benzaldehyde | in 100 mM glycine-NaOH (pH 9.2), at 25°C | Pseudomonas putida | |
1.2.1.85 | 0.53 | - |
4-methylbenzaldehyde | in 100 mM glycine-NaOH (pH 9.2), at 25°C | Pseudomonas putida | |
1.2.1.85 | 0.63 | - |
3-methylbenzaldehyde | in 100 mM glycine-NaOH (pH 9.2), at 25°C | Pseudomonas putida | |
1.2.1.85 | 0.81 | - |
3-Nitrobenzaldehyde | in 100 mM glycine-NaOH (pH 9.2), at 25°C | Pseudomonas putida | |
1.2.1.85 | 0.94 | - |
3-Methoxybenzaldehyde | in 100 mM glycine-NaOH (pH 9.2), at 25°C | Pseudomonas putida | |
1.2.1.85 | 1.3 | - |
3-Chlorobenzaldehyde | in 100 mM glycine-NaOH (pH 9.2), at 25°C | Pseudomonas putida |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.2.1.28 | 51941 | - |
4 * 51941, deduced from nucleotide sequence | Pseudomonas putida |
1.2.1.28 | 52500 | - |
4 * 52500, SDS-PAGE | Pseudomonas putida |
1.2.1.28 | 122000 | 126000 | gel filtration | Pseudomonas putida |
1.2.1.28 | 208000 | - |
gel filtration | Pseudomonas putida |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.28 | benzaldehyde + NAD+ + H2O | Pseudomonas putida | - |
benzoate + NADH + H+ | - |
? | |
1.2.1.28 | benzaldehyde + NAD+ + H2O | Pseudomonas putida KT 2240 | - |
benzoate + NADH + H+ | - |
? | |
1.2.1.32 | 2-hydroxymuconic acid semialdehyde + NAD+ + H2O | Pseudomonas putida | - |
2-hydroxymuconate + NADH + H+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.1.28 | Pseudomonas putida | - |
- |
- |
1.2.1.32 | Pseudomonas putida | - |
- |
- |
1.2.1.85 | Pseudomonas putida | - |
- |
- |
1.2.1.85 | Pseudomonas putida KT 2240 | - |
- |
- |
1.13.11.2 | Pseudomonas putida | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.2.1.28 | - |
Pseudomonas putida |
1.2.1.32 | erroneous identification as benzaldehyde dehydrogenase corrected | Pseudomonas putida |
1.2.1.85 | ammonium sulfate fractionation, TSKgel DEAE-5PW column chromatography, Affigel-Blue affinity chromatography, and TSKgel Phenyl-5PW column chromatography | Pseudomonas putida |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.2.1.28 | 16 | - |
- |
Pseudomonas putida |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.28 | 3-chlorobenzaldehyde + NAD+ + H2O | - |
Pseudomonas putida | 3-chlorobenzoate + NADH | - |
? | |
1.2.1.28 | 3-chlorobenzaldehyde + NAD+ + H2O | - |
Pseudomonas putida KT 2240 | 3-chlorobenzoate + NADH | - |
? | |
1.2.1.28 | 3-fluorobenzaldehyde + NAD+ + H2O | - |
Pseudomonas putida | 3-fluorobenzoate + NADH + H+ | - |
? | |
1.2.1.28 | 3-methoxybenzaldehyde + NAD+ + H2O | - |
Pseudomonas putida | 3-methoxybenzoate + NADH | - |
? | |
1.2.1.28 | 3-methylbenzaldehyde + NAD+ + H2O | - |
Pseudomonas putida | 3-methylbenzoate + NADH | - |
? | |
1.2.1.28 | 3-nitrobenzaldehyde + NAD+ + H2O | - |
Pseudomonas putida | 3-nitrobenzoate + NADH | - |
? | |
1.2.1.28 | 3-nitrobenzaldehyde + NAD+ + H2O | - |
Pseudomonas putida KT 2240 | 3-nitrobenzoate + NADH | - |
? | |
1.2.1.28 | 4-carboxybenzaldehyde + NAD+ + H2O | 1650% of activity with benzaldehyde | Pseudomonas putida | 4-carboxybenzoate + NADH | - |
? | |
1.2.1.28 | 4-carboxybenzaldehyde + NAD+ + H2O | 1650% of activity with benzaldehyde | Pseudomonas putida KT 2240 | 4-carboxybenzoate + NADH | - |
? | |
1.2.1.28 | 4-methoxybenzaldehyde + NAD+ + H2O | 90% of activity with benzaldehyde | Pseudomonas putida | 4-methoxybenzoate + NADH + H+ | - |
? | |
1.2.1.28 | 4-methylbenzaldehyde + NAD+ + H2O | - |
Pseudomonas putida | 4-methylbenzoate + NADH | - |
? | |
1.2.1.28 | benzaldehyde + NAD+ + H2O | - |
Pseudomonas putida | benzoate + NADH + H+ | - |
? | |
1.2.1.28 | benzaldehyde + NAD+ + H2O | - |
Pseudomonas putida | benzoate + NADH + H+ | - |
ir | |
1.2.1.28 | benzaldehyde + NAD+ + H2O | - |
Pseudomonas putida KT 2240 | benzoate + NADH + H+ | - |
? | |
1.2.1.28 | benzaldehyde + NAD+ + H2O | - |
Pseudomonas putida KT 2240 | benzoate + NADH + H+ | - |
ir | |
1.2.1.28 | pyridine 4-carboxyaldehyde + NAD+ + H2O | 1380% of activity with benzaldehyde | Pseudomonas putida | pyridine 4-formiate + NADH | - |
? | |
1.2.1.32 | 2-hydroxy-5-methyl-6-oxohexa-2,4-dienoate + NAD+ + H2O | synonym 5-methyl-2-hydroxymuconic-6-semialdehyde | Pseudomonas putida | 2-hydroxy-5-methylmuconate + NADH | - |
? | |
1.2.1.32 | 2-hydroxymuconate-6-semialdehyde + NAD+ + H2O | - |
Pseudomonas putida | 2-hydroxymuconate + NADH | - |
? | |
1.2.1.32 | 2-hydroxymuconic acid semialdehyde + NAD+ + H2O | - |
Pseudomonas putida | 2-hydroxymuconate + NADH + H+ | - |
? | |
1.2.1.32 | 3-chlorobenzaldehyde + NAD+ + H2O | low activity | Pseudomonas putida | 3-chlorobenzoate + NADH | - |
? | |
1.2.1.32 | 3-fluorobenzaldehyde + NAD+ + H2O | low activity | Pseudomonas putida | 3-fluorobenzoate + NADH + H+ | - |
? | |
1.2.1.32 | 3-methoxybenzaldehyde + NAD+ + H2O | low activity | Pseudomonas putida | 3-methoxybenzoate + NADH | - |
? | |
1.2.1.32 | 3-methylbenzaldehyde + NAD+ + H2O | low activity | Pseudomonas putida | 3-methylbenzoate + NADH | - |
? | |
1.2.1.32 | 3-nitrobenzaldehyde + NAD+ + H2O | low activity | Pseudomonas putida | 3-nitrobenzoate + NADH | - |
? | |
1.2.1.32 | 4-methylbenzaldehyde + NAD+ + H2O | low activity | Pseudomonas putida | 4-methylbenzoate + NADH | - |
? | |
1.2.1.32 | benzaldehyde + NAD+ + H2O | low activity | Pseudomonas putida | benzoate + NADH + H+ | - |
? | |
1.2.1.85 | 2-hydroxy-5-methyl-6-oxohexa-2,4-dienoate + NAD+ + H2O | - |
Pseudomonas putida | (2E,4Z)-2-hydroxy-5-methylhexa-2,4-diendioate + NADH + H+ | - |
? | |
1.2.1.85 | 2-hydroxymuconate-6-semialdehyde + NAD+ + H2O | - |
Pseudomonas putida | (2Z,4E)-2-hydroxyhexa-2,4-dienedioate + NADH + H+ | - |
? | |
1.2.1.85 | 3-chlorobenzaldehyde + NAD+ + H2O | - |
Pseudomonas putida | 3-chlorobenzoate + NADH + H+ | - |
? | |
1.2.1.85 | 3-fluorobenzaldehyde + NAD+ + H2O | - |
Pseudomonas putida | 3-fluorobenzoate + NADH + H+ | - |
? | |
1.2.1.85 | 3-methoxybenzaldehyde + NAD+ + H2O | - |
Pseudomonas putida | 3-methoxybenzoate + NADH + H+ | - |
? | |
1.2.1.85 | 3-methylbenzaldehyde + NAD+ + H2O | - |
Pseudomonas putida | 3-methylbenzoate + NADH + H+ | - |
? | |
1.2.1.85 | 3-nitrobenzaldehyde + NAD+ + H2O | - |
Pseudomonas putida | 3-nitrobenzoate + NADH + H+ | - |
? | |
1.2.1.85 | 4-methylbenzaldehyde + NAD+ + H2O | - |
Pseudomonas putida | 4-methylbenzoate + NADH + H+ | - |
? | |
1.2.1.85 | benzaldehyde + NAD+ + H2O | weak substrate | Pseudomonas putida | benzoate + NADH + H+ | - |
? | |
1.13.11.2 | 4-methylcatechol + O2 | - |
Pseudomonas putida | 2-hydroxy-5-methyl-6-oxohexa-2,4-dienoate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.2.1.28 | tetramer | 4 * 52500, SDS-PAGE | Pseudomonas putida |
1.2.1.28 | tetramer | 4 * 51941, deduced from nucleotide sequence | Pseudomonas putida |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.2.1.85 | 2-hydroxymuconic semialdehyde dehydrogenase | - |
Pseudomonas putida |
1.2.1.85 | XylG | - |
Pseudomonas putida |
1.13.11.2 | XylE | - |
Pseudomonas putida |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.2.1.28 | 9 | - |
- |
Pseudomonas putida |
1.2.1.85 | 8.3 | - |
pH optimum for the oxidation of 2-hydroxymuconic semialdehyde | Pseudomonas putida |
1.2.1.85 | 9.6 | - |
pH optimum for the oxidation of benzaldehyde | Pseudomonas putida |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.2.1.28 | 6 | 10 | - |
Pseudomonas putida |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.28 | NAD+ | - |
Pseudomonas putida | |
1.2.1.32 | NAD+ | - |
Pseudomonas putida |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.1.85 | 8 | - |
3-Methoxybenzaldehyde | apparent value, in 100 mM glycine-NaOH (pH 9.2), at 25°C | Pseudomonas putida | |
1.2.1.85 | 10 | - |
4-methylbenzaldehyde | apparent value, in 100 mM glycine-NaOH (pH 9.2), at 25°C | Pseudomonas putida | |
1.2.1.85 | 20 | - |
3-Nitrobenzaldehyde | apparent value, in 100 mM glycine-NaOH (pH 9.2), at 25°C | Pseudomonas putida | |
1.2.1.85 | 22 | - |
3-Chlorobenzaldehyde | apparent value, in 100 mM glycine-NaOH (pH 9.2), at 25°C | Pseudomonas putida | |
1.2.1.85 | 32 | - |
benzaldehyde | apparent value, in 100 mM glycine-NaOH (pH 9.2), at 25°C | Pseudomonas putida | |
1.2.1.85 | 36 | - |
3-methylbenzaldehyde | apparent value, in 100 mM glycine-NaOH (pH 9.2), at 25°C | Pseudomonas putida | |
1.2.1.85 | 56 | - |
NAD+ | apparent value, in the presence of benzaldehyde, 100 mM glycine-NaOH (pH 9.2), at 25°C | Pseudomonas putida | |
1.2.1.85 | 330 | - |
3-Fluorobenzaldehyde | apparent value, in 100 mM glycine-NaOH (pH 9.2), at 25°C | Pseudomonas putida | |
1.2.1.85 | 1600 | - |
2-hydroxymuconate-6-semialdehyde | apparent value, in 100 mM Tris-HCl, pH 8.5, at 25°C | Pseudomonas putida | |
1.2.1.85 | 2100 | - |
2-hydroxy-5-methyl-6-oxohexa-2,4-dienoate | apparent value, in 100 mM Tris-HCl, pH 8.5, at 25°C | Pseudomonas putida |