Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kedishvili, N.Y.; Goodwin, G.W.; Popov, K.M.; Harris, R.A.
    Mammalian methylmalonate-semialdehyde dehydrogenase (2000), Methods Enzymol., 324, 207-218.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.27
-
 Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
1.2.1.27 C285A expressed in Escherichia coli, no activity Rattus norvegicus
1.2.1.27 N251E expressed in Escherichia coli, no activity Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.27 0.0025
-
 2-Methyl-3-oxopropanoate
-
 Rattus norvegicus
1.2.1.27 0.0045
-
 malonate semialdehyde
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.1.27 mitochondrion mitochondrial targeting sequence Rattus norvegicus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.27 55330
-
 4 * 55330, cDNA sequence Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.27 Homo sapiens
-
-
-
1.2.1.27 Rattus norvegicus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.2.1.27 side-chain modification acylation of active site cysteine residue Cys-285 by long-chain fatty acyl-CoA esters inactivates the enzyme Rattus norvegicus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.27
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.27 2-methyl-3-oxopropanoate + CoA + NAD+
-
 Rattus norvegicus propanoyl-CoA + CO2 + NADH
-
 ir
1.2.1.27 malonate semialdehyde + CoA + NAD+
-
 Rattus norvegicus acetyl-CoA + CO2 + NADH
-
 ?
1.2.1.27 p-nitrophenyl acetate + CoA + NAD+
-
 Rattus norvegicus p-nitrophenol + acetyl-CoA + NADH
-
 ir

Subunits

EC Number Subunits Comment Organism
1.2.1.27 tetramer 4 * 55330, cDNA sequence Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.27 CoA
-
 Homo sapiens
1.2.1.27 CoA
-
 Rattus norvegicus
1.2.1.27 NAD+
-
 Homo sapiens
1.2.1.27 NAD+
-
 Rattus norvegicus