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Literature summary extracted from

  • Boronat, A.; Caballero, E.; Aguilar, J.
    Experimental evolution of a metabolic pathway for ethylene glycol utilization by Escherichia coli (1983), J. Bacteriol., 153, 134-139.
    View publication on PubMedView publication on EuropePMC

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.21 Escherichia coli
-
grown on ethylene glycol
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.21 0.145
-
enzyme activity in crude extracts of wild-type strain G1 Escherichia coli
1.2.1.21 0.165
-
enzyme activity in crude extracts of wild-type strain G3 Escherichia coli
1.2.1.21 0.56
-
enzyme activity in crude extracts of ethylene glycol positive mutant strain EG3 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.21 glycolaldehyde + NAD+ + H2O
-
Escherichia coli glycolate + NADH
-
?

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.21 NAD+
-
Escherichia coli
1.2.1.21 NADP+ 10 times lower activity compared to NAD+ Escherichia coli