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Literature summary extracted from

  • Moore, R.A.; Bocik, W.E.; Viola, R.E.
    Expression and purification of aspartate beta-semialdehyde dehydrogenase from infectious microorganisms (2002), Protein Expr. Purif., 25, 189-194.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.11 expressed as inclusion bodies with an N-terminal hexahistidine tag in pET28b vector or subcloned into pET41 vector with NdeI/SalI Helicobacter pylori
1.2.1.11 expressed in Escherichia coli Haemophilus influenzae
1.2.1.11 expressed in Escherichia coli Pseudomonas aeruginosa
1.2.1.11 expressed in Escherichia coli, the genome contains two genes for the enzyme Vibrio cholerae serotype O1

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.11 0.12
-
 L-aspartate 4-semialdehyde
-
 Pseudomonas aeruginosa
1.2.1.11 0.13
-
 NADP+
-
 Pseudomonas aeruginosa
1.2.1.11 0.15
-
 NADP+
-
 Haemophilus influenzae
1.2.1.11 0.16
-
 L-aspartate 4-semialdehyde gene II Vibrio cholerae serotype O1
1.2.1.11 0.19
-
 L-aspartate 4-semialdehyde gene I Vibrio cholerae serotype O1
1.2.1.11 0.24
-
 L-aspartate 4-semialdehyde
-
 Haemophilus influenzae
1.2.1.11 0.32
-
 NADP+ gene I Vibrio cholerae serotype O1
1.2.1.11 0.36
-
 NADP+ gene II Vibrio cholerae serotype O1
1.2.1.11 1.1
-
 phosphate gene I Vibrio cholerae serotype O1
1.2.1.11 1.5
-
 phosphate
-
 Pseudomonas aeruginosa
1.2.1.11 1.6
-
 phosphate
-
 Haemophilus influenzae
1.2.1.11 22
-
 phosphate gene II Vibrio cholerae serotype O1

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.11 L-4-aspartyl phosphate + NADPH Haemophilus influenzae
-
 L-aspartate 4-semialdehyde + phosphate + NADP+
-
 ?
1.2.1.11 L-4-aspartyl phosphate + NADPH Vibrio cholerae serotype O1
-
 L-aspartate 4-semialdehyde + phosphate + NADP+
-
 ?
1.2.1.11 L-4-aspartyl phosphate + NADPH Pseudomonas aeruginosa
-
 L-aspartate 4-semialdehyde + phosphate + NADP+
-
 ?
1.2.1.11 L-4-aspartyl phosphate + NADPH Helicobacter pylori
-
 L-aspartate 4-semialdehyde + phosphate + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.11 Haemophilus influenzae
-
-
-
1.2.1.11 Helicobacter pylori O25801
-
-
1.2.1.11 Pseudomonas aeruginosa Q51344
-
-
1.2.1.11 Vibrio cholerae P23247
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.11
-
 Helicobacter pylori
1.2.1.11 using ammonium sulfate precipitation and column chromatography on Q Sepharose XL and Procion Red-A Haemophilus influenzae
1.2.1.11 using column chromatography on Q Sepharose XL and omega-aminohexyl-agarose for the purification of V. cholerae I and column chromatography on Q Sepharose XL for the purification of V. cholerae II Vibrio cholerae serotype O1
1.2.1.11 using column chromatography on Q Sepharose XL and phenyl-Sepharose Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.11 additional information
-
-
 Haemophilus influenzae
1.2.1.11 additional information
-
-
 Vibrio cholerae serotype O1
1.2.1.11 additional information
-
-
 Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.11 L-4-aspartyl phosphate + NADPH
-
 Haemophilus influenzae L-aspartate 4-semialdehyde + phosphate + NADP+
-
 ?
1.2.1.11 L-4-aspartyl phosphate + NADPH
-
 Vibrio cholerae serotype O1 L-aspartate 4-semialdehyde + phosphate + NADP+
-
 ?
1.2.1.11 L-4-aspartyl phosphate + NADPH
-
 Pseudomonas aeruginosa L-aspartate 4-semialdehyde + phosphate + NADP+
-
 ?
1.2.1.11 L-4-aspartyl phosphate + NADPH
-
 Helicobacter pylori L-aspartate 4-semialdehyde + phosphate + NADP+
-
 ?
1.2.1.11 L-aspartate 4-semialdehyde + phosphate + NADP+
-
 Haemophilus influenzae L-4-aspartyl phosphate + NADPH
-
 r
1.2.1.11 L-aspartate 4-semialdehyde + phosphate + NADP+
-
 Vibrio cholerae serotype O1 L-4-aspartyl phosphate + NADPH
-
 r
1.2.1.11 L-aspartate 4-semialdehyde + phosphate + NADP+
-
 Pseudomonas aeruginosa L-4-aspartyl phosphate + NADPH
-
 r
1.2.1.11 L-aspartate 4-semialdehyde + phosphate + NADP+
-
 Helicobacter pylori L-4-aspartyl phosphate + NADPH
-
 r

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.1.11 58
-
 L-aspartate 4-semialdehyde gene II Vibrio cholerae serotype O1
1.2.1.11 120
-
 L-aspartate 4-semialdehyde gene I Vibrio cholerae serotype O1
1.2.1.11 160
-
 L-aspartate 4-semialdehyde
-
 Pseudomonas aeruginosa
1.2.1.11 330
-
 L-aspartate 4-semialdehyde
-
 Haemophilus influenzae

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.11 NADP+
-
 Haemophilus influenzae
1.2.1.11 NADP+
-
 Vibrio cholerae serotype O1
1.2.1.11 NADP+
-
 Pseudomonas aeruginosa
1.2.1.11 NADP+
-
 Helicobacter pylori