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Literature summary extracted from
- Microsomal cytochrome P-450 from neonatal pig testis. Purification and properties of A C21 steroid side-chain cleavage system (17alpha-hydroxylase-C17,20 lyase) (1981), J. Biol. Chem., 256, 3871-3876.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.14.19 | 0.0015 | - |
progesterone | - |
Sus scrofa |  |
| 1.14.14.19 | 0.0024 | - |
17alpha-hydroxyprogesterone | - |
Sus scrofa | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.14.19 | microsome | membrane-bound | Sus scrofa | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.19 | Fe3+ | 8 nM per mg protein | Sus scrofa | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.19 | 59000 | - |
SDS-PAGE and denaturating gel filtration | Sus scrofa |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.14.19 | Sus scrofa | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 1.14.14.19 | glycoprotein | - |
Sus scrofa |
| 1.14.14.19 | phospholipoprotein | 40 nmol phospholipid per mg of protein | Sus scrofa |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.14.19 | - |
Sus scrofa |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.14.19 | testis | - |
Sus scrofa | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.19 | 0.008 | - |
lyase activity | Sus scrofa |
| 1.14.14.19 | 0.023 | - |
hydroxylase activity | Sus scrofa |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.14.14.19 | -70°C, 0.2% Emulgen 913 v/v and 20% glycerol v/v, 6 months, no detectable loss of activity | Sus scrofa |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.14.19 | monomer | alpha, 1 * 59000 | Sus scrofa |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.19 | 37 | - |
- |
Sus scrofa |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.19 | 7.3 | - |
- |
Sus scrofa |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.19 | NADPH | - |
Sus scrofa | |
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Release 2023.1 (January 2023)
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