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Literature summary extracted from

  • Beaudoin, C.; Lavallee, B.; Tremblay, Y.; Hu, D.W.; Breton, R.; De Launoit, Y.; Belanger, A.
    Modulation of 17alpha-hydroxylase/17,20-lyase activity of guinea pig cytochrome P450c17 by site-directed mutagenesis (1998), DNA Cell Biol., 17, 707-715.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.19 expression in monkey kidney COS-1 cells Cavia porcellus
1.14.14.19 expression in monkey kidney COS-1 cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.14.14.19 R200N increased reactivity towards pregnenolone, converts pregnenolone to 17alpha-hydroxypregnenolone and dehydroepiandrosterone, at the expense of 17,20-lyase activity towards 17alpha-hydroxyprogesterone Cavia porcellus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.19 0.00008
-
 pregnenolone wild type enzyme Cavia porcellus
1.14.14.19 0.00022
-
 pregnenolone R200N mutant Cavia porcellus
1.14.14.19 0.00024
-
 progesterone wild type enzyme Cavia porcellus
1.14.14.19 0.00025
-
 pregnenolone
-
 Homo sapiens
1.14.14.19 0.00031
-
 progesterone R200N mutant Cavia porcellus
1.14.14.19 0.00045
-
 progesterone
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.14.19 microsome
-
 Homo sapiens
-
-
1.14.14.19 microsome membrane-bound Cavia porcellus
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.19 progesterone + [reduced NADPH-hemoprotein reductase] + O2 Cavia porcellus
-
 17alpha-hydroxyprogesterone + [oxidized NADPH-hemoprotein reductase] + H2O product eliminates at C20,21 acetate to yield androstenedione ?
1.14.14.19 progesterone + [reduced NADPH-hemoprotein reductase] + O2 Homo sapiens
-
 17alpha-hydroxyprogesterone + [oxidized NADPH-hemoprotein reductase] + H2O product eliminates at C20,21 acetate to yield androstenedione ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.19 Cavia porcellus
-
-
-
1.14.14.19 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.19 progesterone + [reduced NADPH-hemoprotein reductase] + O2
-
 Cavia porcellus 17alpha-hydroxyprogesterone + [oxidized NADPH-hemoprotein reductase] + H2O product eliminates at C20,21 acetate to yield androstenedione ?
1.14.14.19 progesterone + [reduced NADPH-hemoprotein reductase] + O2
-
 Homo sapiens 17alpha-hydroxyprogesterone + [oxidized NADPH-hemoprotein reductase] + H2O product eliminates at C20,21 acetate to yield androstenedione ?