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Literature summary extracted from

  • Watanabe, T.; Kambe, H.; Imamura, I.; Taguchi, Y.; Tamura, T.; Wada, H.
    Method for enzymatic determination of imidazole acetic acid (1983), Anal. Biochem., 130, 321-327.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.5 5-hydroxyindoleacetate 11% inhibition at 4.5 mM Pseudomonas sp.
1.14.13.5 dihydroxymandelate 53% inhibition at 4.5 mM Pseudomonas sp.
1.14.13.5 Dihydroxyphenylacetate 66% inhibition at 4.5 mM Pseudomonas sp.
1.14.13.5 homogentisate 50% inhibition at 4.5 mM Pseudomonas sp.
1.14.13.5 homovanillic acid 5% inhibition at 4.5 mM Pseudomonas sp.
1.14.13.5 Imidazolepyruvate 56% inhibition at 4.5 mM Pseudomonas sp.
1.14.13.5 indoleacetate 22% inhibition at 4.5 mM Pseudomonas sp.
1.14.13.5 m-hydroxyphenylacetate 79% inhibition at 4.5 mM Pseudomonas sp.
1.14.13.5 N-methylimidazoleacetate 11% inhibition at 4.5 mM Pseudomonas sp.
1.14.13.5 p-hydroxybenzoate 38% inhibition at 4.5 mM Pseudomonas sp.
1.14.13.5 p-hydroxyphenylacetate 47% inhibition at 4.5 mM Pseudomonas sp.
1.14.13.5 phenylacetate 25% inhibition at 4.5 mM Pseudomonas sp.
1.14.13.5 vanillyl mandelic acid 9% inhibition at 4.5 mM Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.5 0.48
-
 imidazoleacetate
-
 Pseudomonas sp.
1.14.13.5 23
-
 5-hydroxyindoleacetate
-
 Pseudomonas sp.
1.14.13.5 44
-
 N-methylimidazoleacetate
-
 Pseudomonas sp.
1.14.13.5 64
-
 indoleacetate
-
 Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.5 4-imidazoleacetate + NADH + O2 Pseudomonas sp.
-
 5-hydroxy-4-imidazoleacetate + NAD+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.5 Pseudomonas sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.5 partial, using ammonium sulfate precipitation and column chromatography on DEAE-Sephadex A-50 Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.5 additional information
-
-
 Pseudomonas sp.

Storage Stability

EC Number Storage Stability Organism
1.14.13.5 -20°C, stable for at least 6 months Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.5 4-imidazoleacetate + NAD(P)H + O2 + H+ highly specific for 4-imidazoleacetate Pseudomonas sp. 5-hydroxy-4-imidazoleacetate + NAD(P)+ + H2O
-
 ?
1.14.13.5 4-imidazoleacetate + NADH + O2
-
 Pseudomonas sp. 5-hydroxy-4-imidazoleacetate + NAD+ + H2O
-
 ?
1.14.13.5 5-hydroxyindoleacetate + NAD(P)H + O2 + H+ 9.4% relative activity to 4-imidazoleacetate Pseudomonas sp. ? + NAD(P)+ + H2O
-
 ?
1.14.13.5 imidazolepropionate + NAD(P)H + O2 + H+ 9.5% relative activity to 4-imidazoleacetate Pseudomonas sp. 5-hydroxyimidazolepropionate + NAD(P)+ + H2O
-
 ?
1.14.13.5 imidazolepyruvate + O2 + NAD(P)H + H+ 2.8% relative activity to 4-imidazoleacetate Pseudomonas sp. 5-hydroxyimidazolepyruvate + NAD(P)+ + H2O
-
 ?
1.14.13.5 indoleacetate + NAD(P)H + O2 + H+ 7.3% relative activity to 4-imidazoleacetate Pseudomonas sp. 5-hydroxyindoleacetate + NAD(P)+ + H2O
-
 ?
1.14.13.5 N-methylimidazoleacetate + NAD(P)H + O2 + H+ 7.6% relative activity to 4-imidazoleacetate Pseudomonas sp. 5-hydroxy-N-methylimidazoleacetate + NAD(P)+ + H2O
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.5 NADH
-
 Pseudomonas sp.