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Literature summary extracted from

  • Gershenzon, J.; McCaskill, D.; Rajaonarivony, J.I.; Mihaliak, C.; Karp, F.; Croteau, R.
    Isolation of secretory cells from plant glandular trichomes and their use in biosynthetic studies of monoterpenes and other gland products (1992), Anal. Biochem., 200, 130-138.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.14.14.51 DTT stabilizes during purification Mentha spicata
1.14.14.51 EDTA stabilizes during purification Mentha spicata
1.14.14.51 glycerol stabilizes during purification Mentha spicata
1.14.14.99 DTT stabilizes during purification Mentha x piperita
1.14.14.99 EDTA stabilizes during purification Mentha x piperita
1.14.14.99 glycerol stabilizes during purification Mentha x piperita

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.14.51 endoplasmic reticulum
-
 Mentha spicata 5783
-
1.14.14.99 endoplasmic reticulum
-
 Mentha x piperita 5783
-

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.51 Mentha spicata
-
 spearmint
-
1.14.14.99 Mentha x piperita
-
 peppermint
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.14.51 partial Mentha spicata
1.14.14.51 sonication of secretory cells leads to most active crude extracts Mentha spicata
1.14.14.99 sonication of secretory cells leads to most active crude extracts Mentha x piperita

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.51 leaf epidermis
-
 Mentha spicata
-
1.14.14.99 leaf epidermis oil gland secretory cells Mentha x piperita
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.14.51 0.0007
-
-
 Mentha spicata
1.14.14.99 0.0003333
-
-
 Mentha x piperita

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.51 (-)-(4S)-limonene + NADPH + O2 highly specific Mentha spicata (-)-(4R,6S)-trans-carveol + NADP+ + H2O
-
 ?
1.14.14.99 (-)-(4S)-limonene + [reduced NADPH-hemoprotein reductase] + O2
-
 Mentha x piperita (-)-trans-(3S,4R)-isopiperitenol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.14.51 7.4
-
-
 Mentha spicata
1.14.14.99 7.4
-
-
 Mentha x piperita

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.99 cytochrome P450
-
 Mentha x piperita