EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.52 | FAD | plus FMN, 0.005 mM each, activation | Perilla frutescens | |
1.14.14.52 | FMN | plus FAD, 0.005 mM each, activation | Perilla frutescens | |
1.14.14.99 | FAD | plus FMN, 0.005 mM each, activation | Mentha x piperita | |
1.14.14.99 | FMN | plus FAD, 0.005 mM each, activation | Mentha x piperita |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.51 | 5,11-Dimethyl-6H-pyrido[4,3-b]-carbazole | - |
Mentha spicata | |
1.14.14.51 | clotrimazole | i.e. 1-[chloro-alpha,alpha-diphenyl]imidazole, mixed-type, strong | Mentha spicata | |
1.14.14.51 | CO | CO:O2 ratio of 9:1, photoreversible | Mentha spicata | |
1.14.14.51 | cytochrome c | strong | Mentha spicata | |
1.14.14.51 | Metyrapone | i.e. 2-methyl-1,2-di-3-pyridyl-1-propanone, moderate | Mentha spicata | |
1.14.14.51 | miconazole | i.e. 1-[2,4-dichloro-beta-([2,4-di-chlorobenzyl]oxy)phenethyl]-imidazole, mixed-type, strong | Mentha spicata | |
1.14.14.51 | additional information | no inhibition: ancymidol, imidazole, up to 5 mM | Mentha spicata | |
1.14.14.51 | NADP+ | 2 mM | Mentha spicata | |
1.14.14.51 | SKF 525A | i.e. 2-diethylaminoethyl-2,2-diphenylvalerate, moderate | Mentha spicata | |
1.14.14.52 | 5,11-Dimethyl-6H-pyrido[4,3-b]carbazole | - |
Perilla frutescens | |
1.14.14.52 | clotrimazole | i.e. 1-[chloro-alpha,alpha-diphenyl]imidazole, mixed-type, weak | Perilla frutescens | |
1.14.14.52 | CO | CO:O2 ratio of 9:1, photoreversible | Perilla frutescens | |
1.14.14.52 | cytochrome c | strong | Perilla frutescens | |
1.14.14.52 | Metyrapone | i.e. 2-methyl-1,2-di-3-pyridyl-1-propanone, moderate | Perilla frutescens | |
1.14.14.52 | miconazole | i.e. 1-[2,4-dichloro-beta-([2,4-di-chlorobenzyl]oxy)phenethyl]-imidazole, mixed-type, weak | Perilla frutescens | |
1.14.14.52 | additional information | no inhibition: ancymidol, imidazole, up to 5 mM | Perilla frutescens | |
1.14.14.52 | NADP+ | 2 mM | Perilla frutescens | |
1.14.14.52 | SKF 525A | i.e. 2-diethyl-aminoethyl-2,2-diphenylvalerate, moderate | Perilla frutescens | |
1.14.14.99 | 5,11-Dimethyl-6H-pyrido[4,3-b]carbazole | - |
Mentha x piperita | |
1.14.14.99 | clotrimazole | i.e. 1-[chloro-alpha,alpha-diphenyl]imidazole, mixed-type, strong | Mentha x piperita | |
1.14.14.99 | CO | CO:O2 ratio of 9:1, photoreversible | Mentha x piperita | |
1.14.14.99 | cytochrome c | strong | Mentha x piperita | |
1.14.14.99 | Metyrapone | i.e. 2-methyl-1,2-di-3-pyridyl-1-propanone, inhibition | Mentha x piperita | |
1.14.14.99 | miconazole | i.e. 1-[2,4-dichloro-beta-([2,4-di-chlorobenzyl]oxy)phenethyl]-imidazole, mixed-type, weak | Mentha x piperita | |
1.14.14.99 | additional information | no inhibition: ancymidol, imidazole, up to 5 mM | Mentha x piperita | |
1.14.14.99 | NADP+ | 2 mM | Mentha x piperita | |
1.14.14.99 | SKF 525A | i.e. 2-diethylaminoethyl-2,2-diphenylvalerate, moderate | Mentha x piperita |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.14.51 | 0.02 | - |
(-)-limonene | - |
Mentha spicata | |
1.14.14.52 | 0.021 | - |
(-)-limonene | - |
Perilla frutescens | |
1.14.14.99 | 0.018 | - |
(-)-limonene | - |
Mentha x piperita |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.14.14.51 | microsome | - |
Mentha spicata | - |
- |
1.14.14.99 | microsome | - |
Mentha x piperita | - |
- |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.51 | (-)-limonene + NADPH + O2 | Mentha spicata | one of the key reactions of oxygenated monoterpenes, biosynthesis of (-)-carvone | (-)-trans-carveol + NADP+ + H2O | - |
? | |
1.14.14.52 | (-)-limonene + NADPH + O2 | Perilla frutescens | one of the key reactions of oxygenated monoterpenes, perillyl aldehyde biosynthesis | perillyl alcohol + NADP+ + H2O | - |
? | |
1.14.14.99 | (-)-limonene + [reduced NADPH-hemoprotein reductase] + O2 | Mentha x piperita | one of the key reactions of oxygenated monoterpenes, biosynthesis of (-)-menthone | (-)-trans-isopiperitenol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.14.51 | Mentha spicata | - |
spearmint | - |
1.14.14.52 | Perilla frutescens | - |
- |
- |
1.14.14.99 | Mentha x piperita | - |
peppermint | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.14.51 | partial | Mentha spicata |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.14.14.52 | (S)-limonene + [reduced NADPH-hemoprotein reductase] + O2 = (-)-perillyl alcohol + [oxidized NADPH-hemoprotein reductase] + H2O | side-chain hydroxylation, mixed-function oxygenase | Perilla frutescens | |
1.14.14.99 | (S)-limonene + [reduced NADPH-hemoprotein reductase] + O2 = (-)-trans-isopiperitenol + [oxidized NADPH-hemoprotein reductase] + H2O | mixed-function oxygenase, ring-hydroxylation | Mentha x piperita |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.14.14.51 | leaf epidermis | oil glands on upper and lower surface | Mentha spicata | - |
1.14.14.52 | leaf epidermis | oil glands on abaxial surface | Perilla frutescens | - |
1.14.14.99 | leaf epidermis | of both surfaces | Mentha x piperita | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.14.51 | 0.00068 | - |
- |
Mentha spicata |
1.14.14.52 | 0.0001567 | - |
- |
Perilla frutescens |
1.14.14.99 | 0.000685 | - |
- |
Mentha x piperita |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.51 | (+)-limonene + NADPH + O2 | hydroxylation at 25% the rate of (-)-limonene | Mentha spicata | (+)-cis-carveol + NADP+ + H2O | - |
? | |
1.14.14.51 | (+)-p-menth-1-ene + NADPH + O2 | i.e. (+)-8,9-dihydrolimonene, hydroxylation at 30% the rate of (-)-limonene | Mentha spicata | (+)-cis-carvotanacetol + NADP+ + H2O | - |
? | |
1.14.14.51 | (-)-(4S)-limonene + NADPH + O2 | highly specific | Mentha spicata | (-)-(4R,6S)-trans-carveol + NADP+ + H2O | - |
? | |
1.14.14.51 | (-)-limonene + NADPH + O2 | one of the key reactions of oxygenated monoterpenes, biosynthesis of (-)-carvone | Mentha spicata | (-)-trans-carveol + NADP+ + H2O | - |
? | |
1.14.14.51 | (-)-p-menth-1-ene + NADPH + O2 | i.e. (-)-8,9-dihydrolimonene, hydroxylation at 74% the rate of (-)-limonene | Mentha spicata | (-)-trans-carvotanacetol + NADP+ + H2O | - |
? | |
1.14.14.51 | additional information | no substrates: isolimonenes, terpinolene, alpha- or beta-phellandrene, alpha- or beta-terpinene, bicyclic monoterpenes: pinene, sabinene, alpha-thujene, p-cymene, cis- or trans-p-menthane, NADH can replace NADPH, but more slowly | Mentha spicata | ? | - |
? | |
1.14.14.52 | (+)-limonene + NADPH + O2 | hydroxylation at the same rate as (-)-limonene | Perilla frutescens | perillyl alcohol + NADP+ + H2O | - |
? | |
1.14.14.52 | (-)-limonene + NADPH + O2 | highly specific | Perilla frutescens | perillyl alcohol + NADP+ + H2O | - |
? | |
1.14.14.52 | (-)-limonene + NADPH + O2 | one of the key reactions of oxygenated monoterpenes, perillyl aldehyde biosynthesis | Perilla frutescens | perillyl alcohol + NADP+ + H2O | - |
? | |
1.14.14.52 | additional information | no substrates: isolimonenes, terpinolene, alpha- or beta-phellandrene, alpha- or beta-terpinene, bicyclic monoterpenes: pinene, sabinene, alpha-thujene, p-cymene, cis- or trans-p-menthane, p-menth-1-ene, i.e. 8,9-dihydrolimonene | Perilla frutescens | ? | - |
? | |
1.14.14.99 | (+)-limonene + [reduced NADPH-hemoprotein reductase] + O2 | hydroxylation at 50% | Mentha x piperita | (+)-trans-isopiperitenol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.99 | (+)-p-menth-1-ene + [reduced NADPH-hemoprotein reductase] + O2 | i.e. (+)-8,9-dihydro-limonene, hydroxylation at 37% the rate of (-)-limonene hydroxylation | Mentha x piperita | (+)-trans-piperitol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.99 | (-)-(4S)-limonene + [reduced NADPH-hemoprotein reductase] + O2 | highly specific, regiospecific and stereospecific reaction | Mentha x piperita | (-)-trans-(3S,4R)-isopiperitenol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.99 | (-)-limonene + [reduced NADPH-hemoprotein reductase] + O2 | one of the key reactions of oxygenated monoterpenes, biosynthesis of (-)-menthone | Mentha x piperita | (-)-trans-isopiperitenol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.99 | (-)-p-menth-1-ene + [reduced NADPH-hemoprotein reductase] + O2 | i.e. (-)-8,9-dihydrolimonene, hydroxylation at 37% the rate of (-)-limonene hydroxylation | Mentha x piperita | (-)-trans-piperitol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.99 | additional information | no substrates: isolimonenes, terpinolene, alpha- or beta-phellandrene, alpha- or beta-terpinene, bicyclic monoterpenes: pinene, sabinene, alpha-thujene, p-cymene, cis- or trans-p-menthane, highly specific, absolute requirement for a reduced pyridine nucleotide and molecular oxygen | Mentha x piperita | ? | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.14.51 | 32 | - |
- |
Mentha spicata |
1.14.14.52 | 32 | - |
- |
Perilla frutescens |
1.14.14.99 | 32 | - |
- |
Mentha x piperita |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.14.51 | 7.4 | - |
- |
Mentha spicata |
1.14.14.52 | 7.8 | - |
- |
Perilla frutescens |
1.14.14.99 | 7.4 | - |
- |
Mentha x piperita |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.14.14.51 | 6.9 | 7.9 | about half-maximal activity at pH 6.9 and 7.9 | Mentha spicata |
1.14.14.52 | 7.3 | 8.3 | about half-maximal activity at pH 7.3 and 8.3 | Perilla frutescens |
1.14.14.99 | 6.9 | 7.9 | about half-maximal activity at pH 6.9 and 7.9 | Mentha x piperita |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.51 | cytochrome P450 | average content of 0.0005 mmol/mg protein | Mentha spicata | |
1.14.14.52 | cytochrome P450 | heme-thiolate protein, 0.2-0.9 nmol per mg protein | Perilla frutescens | |
1.14.14.52 | NADH | 5.3% as effective as NADPH | Perilla frutescens | |
1.14.14.52 | NADPH | - |
Perilla frutescens | |
1.14.14.99 | cytochrome P450 | heme-thiolate protein, 0.2-0.9 nmol per mg protein | Mentha x piperita |