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Literature summary extracted from

  • Kikuta, Y.; Kusunose, E.; Sumimoto, H.; Mizukami, Y.; Takeshige, K.; Sakaki, T.; Yabusaki, Y.; Kusunose, M.
    Purification and characterization of recombinant human neutrophil leukotriene B4 omega-hydroxylase (cytochrome P450 4F3) (1998), Arch. Biochem. Biophys., 355, 201-205.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.94 expression in yeast cells Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.14.94 CO recombinant protein, expressed in yeast Homo sapiens
1.14.14.94 Metyrapone recombinant protein, expressed in yeast, 35% inhibition at 2 mM Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.94 0.0006
-
 leukotriene B4 recombinant protein CYP4F3, expressed in yeast Homo sapiens
1.14.14.94 0.06
-
 leukotriene B4 recombinant protein CYP4F2, expressed in yeast Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.14.94 microsome
-
 Homo sapiens
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.14.94 55000
-
 recombinant CYP4F3, expressed in yeast, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.94 (6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate + [reduced NADPH-hemoprotein reductase] + O2 Homo sapiens inactivation of leukotriene B4, a mediator of inflammation (6Z,8E,10E,14Z)-(5S,12R)-5,12,20-trihydroxyicosa-6,8,10,14-tetraenoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.94 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.14.94 recombinant protein CYP4F3, expressed in yeast Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.94 neutrophil
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.14.94 30
-
 recombinant protein, expressed in yeast Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.94 (6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate + [reduced NADPH-hemoprotein reductase] + O2 inactivation of leukotriene B4, a mediator of inflammation Homo sapiens (6Z,8E,10E,14Z)-(5S,12R)-5,12,20-trihydroxyicosa-6,8,10,14-tetraenoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.94 leukotriene B4 + [reduced NADPH-hemoprotein reductase] + O2 leukotriene B4 is identical with (6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate Homo sapiens 20-hydroxy-leukotriene B4 + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.94 additional information recombinant protein CYP4F3, wide substrate specificity Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.94 CYP4F3
-
 Homo sapiens
1.14.14.94 cytochrome P450 4F3
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.14.94 7.5
-
 recombinant protein, expressed in yeast Homo sapiens