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Literature summary extracted from

  • Chen, R.; Oki, H.; Scott, R.P.; Yamaguchi, H.; Kusunoki, M.; Matsuura, Y.; Chaen, H.; Tsugita, A.; Hosokawa, K.
    Crystallization and further characterization of meta-hydroxybenzoate 4-hydroxylase from Comamonas testosteroni (1998), Res. Commun. Biochem. Cell Mol. Biol., 2, 253-274.
No PubMed abstract available

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.13.2
-
Comamonas testosteroni
1.14.13.23 1.4-1.6 M ammonium sulfate, 4-8% dioxane, pH 6.5 Comamonas testosteroni

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.2 iodoacetamide reversed by dithiothreitol Comamonas testosteroni
1.14.13.2 N-iodosuccinimide reversed by dithiothreitol Comamonas testosteroni
1.14.13.2 o-Iodosobenzoate reversed by dithiothreitol Comamonas testosteroni
1.14.13.2 p-hydroxy-3-iodomethylbenzoate 1 mM, irreversible crosslinking to the substrate binding site Comamonas testosteroni
1.14.13.23 4-hydroxy-3-iodomethylbenzoate inhibition not reversed in presence of dithiotreitol Comamonas testosteroni
1.14.13.23 iodoacetamide inhibition reversed in presence of dithiotreitol Comamonas testosteroni
1.14.13.23 N-iodosuccinimide inhibition reversed in presence of dithiotreitol Comamonas testosteroni
1.14.13.23 o-Iodosobenzoate inhibition reversed in presence of dithiotreitol Comamonas testosteroni

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.2 71000
-
x * 71000, SDS-PAGE Comamonas testosteroni
1.14.13.23 71000
-
x * 71000, SDS-PAGE Comamonas testosteroni

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.23 3-hydroxybenzoate + [reduced NADPH-hemoprotein reductase] + O2 Comamonas testosteroni
-
3,4-dihydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.2 Comamonas testosteroni
-
-
-
1.14.13.23 Comamonas testosteroni
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.2
-
Comamonas testosteroni

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.23 6.03
-
-
Comamonas testosteroni

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.2 4-hydroxybenzoate + NADPH + O2
-
Comamonas testosteroni protocatechuate + NADP+ + H2O
-
?
1.14.13.23 3-hydroxybenzoate + [reduced NADPH-hemoprotein reductase] + O2
-
Comamonas testosteroni 3,4-dihydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
1.14.13.23 gentisate + NADPH + O2
-
Comamonas testosteroni ?
-
?

Subunits

EC Number Subunits Comment Organism
1.14.13.2 ? x * 71000, SDS-PAGE Comamonas testosteroni
1.14.13.23 ? x * 71000, SDS-PAGE Comamonas testosteroni

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.23 6.2
-
in potassium phosphate buffer Comamonas testosteroni
1.14.13.23 7.3
-
in Tris-HCl buffer Comamonas testosteroni

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.23 FAD flavoprotein Comamonas testosteroni
1.14.13.23 NADPH specific requirement for NADPH Comamonas testosteroni