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Literature summary extracted from
- Purification and characterization of 4-hydroxybenzoate 3-hydroxylase from a Klebsiella pneumoniae mutant strain (1995), Arch. Microbiol., 164, 70-77.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.2 | diethyl dicarbonate | - |
Klebsiella pneumoniae |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.13.2 | 0.022 | - |
4-hydroxybenzoate | - |
Klebsiella pneumoniae | |
| 1.14.13.2 | 0.025 | - |
NADPH | - |
Klebsiella pneumoniae | |
| 1.14.13.2 | 0.037 | - |
O2 | - |
Klebsiella pneumoniae | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.2 | 40000 | - |
2 * 40000, SDS-PAGE | Klebsiella pneumoniae |
| 1.14.13.2 | 80000 | - |
gel filtration | Klebsiella pneumoniae |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.13.2 | 4-hydroxybenzoate + NADPH + O2 | Klebsiella pneumoniae | enzyme is expressed at basal level, presence of 4-hydroxybenzoate enhances activity | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.13.2 | Klebsiella pneumoniae | - |
enzyme is expressed in mutant strain MAO4, but not in wild-type strain | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.13.2 | - |
Klebsiella pneumoniae |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.2 | 5.76 | - |
- |
Klebsiella pneumoniae |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.14.13.2 | 4°C, 50 mM phosphate buffer, 50 mM Tris-HCl, pH 8.0, 10% glycerol, 10 mM EDTA, loss of activity after 72 h | Klebsiella pneumoniae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.13.2 | 4-hydroxybenzoate + NADPH + O2 | - |
Klebsiella pneumoniae | protocatechuate + NADP+ + H2O | - |
? | |
| 1.14.13.2 | 4-hydroxybenzoate + NADPH + O2 | enzyme is expressed at basal level, presence of 4-hydroxybenzoate enhances activity | Klebsiella pneumoniae | ? | - |
? | |
| 1.14.13.2 | additional information | a Tyr seems to be involved in substrate activation | Klebsiella pneumoniae | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.13.2 | dimer | 2 * 40000, SDS-PAGE | Klebsiella pneumoniae |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.2 | 25 | 30 | - |
Klebsiella pneumoniae |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.2 | 4 | - |
50 mM phosphate buffer, 50 mM Tris-HCl, pH 8.0, 10% glycerol, 10 mM EDTA, loss of activity after 72 h | Klebsiella pneumoniae |
| 1.14.13.2 | 21 | - |
room temperature, without glycerol and EDTA, complete inactivation after 20 h | Klebsiella pneumoniae |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.2 | 8 | - |
- |
Klebsiella pneumoniae |
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