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Literature summary extracted from

  • Moran, G.R.; Entsch, B.; Palfey, B.A.; Ballou, D.P.
    Mechanistic insights into p-hydroxybenzoate hydroxylase from studies of the mutant Ser212Ala (1999), Biochemistry, 38, 6292-6299.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.2 expression in Escherichia coli, mutant enzyme S212A Pseudomonas aeruginosa

Protein Variants

EC Number Protein Variants Comment Organism
1.14.13.2 S212A the turnover of the substrate 2,4-dihydroxybenzoate is 1.5-fold faster than the rate observed with the wild-type Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.2 Pseudomonas aeruginosa P20586
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.2 2,4-dihydroxybenzoate + NADPH + O2
-
 Pseudomonas aeruginosa 2,3,4-trihydroxybenzoate + 2,4,5-trihydroxybenzoate + NADP+ + H2O
-
 ?
1.14.13.2 4-hydroxybenzoate + NADPH + O2
-
 Pseudomonas aeruginosa protocatechuate + NADP+ + H2O
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.2 FAD movement of the flavin occurs before reduction Pseudomonas aeruginosa